【作者】 张玉霞；

【导师】 陈宏丽；

【作者基本信息】 兰州大学 ， 分析化学， 2007， 硕士

【摘要】 藏医药的物质基础是青藏高原特有的高原植物。藏药植物的特征是强辐射、日照时间长，决定了其光合作用强，有效成分的积累高，生物活性强。目前，对于藏药的质量分析和鉴别还很有限，不能保证藏药的质量和开发新藏药的研究。毛细管电泳是一种新型分离分析技术，具有高效、快速、进样体积小、可选择的分离模式多特点，但由于它的检测灵敏度不如高效液相色谱，而藏药植物中活性成分的含量又很低，导致毛细管电泳在藏药活性组分的分析方面应用很少。鉴于这种情况，本论文从理论和方法上开展了毛细管电泳在藏药活性组分分离测定方面的研究工作。本论文中第二部分就充分利用了毛细管电泳的优势，使其应用于藏药湿生扁蕾全草中活性组分的分离与测定，取得了以下几方面创新性的研究成果：1．首次建立了分离1，7-二羟基-3，8-二甲氧基（口山）酮和1，8-二羟基-3，7-二甲氧基（口山）酮的胶束电动毛细管电泳新方法，并将该方法成功地用于藏药湿生扁蕾中这两种（口山）酮的含量测定。2．首次将非水毛细管电泳法用于藏药湿生扁蕾中熊果酸和齐墩果酸的分离与测定，结果令人满意。本论文共分为三部分四章：第一部分：综述第一章：简单介绍了CE的原理和特点、藏医药的研究现状以及毛细管电泳在（口山）酮类化合物和萜类化合物中的应用。第二部分：毛细管电泳应用于藏药湿生扁蕾中活性成分的分离与测定第二章：首次建立了同时分离测定藏药湿生扁蕾全草花和杆中的1，7-二羟基-3，8-二甲氧基（口山）酮（BL1）和1，8-二羟基-3，7-二甲氧基（口山）酮（BL2）的毛细管胶束电动色谱新方法。最佳分析条件为：20 mM硼砂+20 mM SDS，pH 9.80，分离电压17.5 kV，检测波长260 nm。该方法不仅可以用于分析藏药湿生扁蕾中的这些活性成分，而且对其它龙胆科扁蕾属植物中这些活性成分的初期研究也具有重要的参考价值。第三章：建立了一种新的非水毛细管电泳方法，可以同时测定藏药湿生扁蕾全草花和杆中的齐墩果酸和熊果酸。最佳分析条件为：25 mM Tris、10％乙腈的甲醇溶液、1.5％醋酸和25 kV的分离电压。发现湿生扁蕾花是提取这些活性组分的有效部位。将该方法进行改善，可用于其它龙胆科扁蕾属植物中这些活性成分的测定。第三部分：自由溶液毛细管电泳在银黄制剂中的应用研究第四章：建立了一种简单、灵敏、快速、准确的自由溶液毛细管电泳新方法，同时测定了银黄口服液、银黄颗粒、银黄片中的两种有效成分绿原酸和黄芩苷。最佳分析条件为：缓冲溶液，n_borate：n_NaH2PO4=1：1，pH=8.0，分离电压为17.5kV，在此最佳分离条件下，黄芩苷和绿原酸在4.3分钟内完全达到基线分离。更多还原

【Abstract】 Particular tableland plants in Qinghai and Tibetan altiplano are substantial foundation of Tibetan medicines. Strong radiation and long sunlight of Tibetan medicines plants leads to doughty photosynthesis, high accumulation of the effective components and strong bioactivities. At present, it exist obvious confine in analysis of quality and distinguishing of Tibetan medicines, which can’t assure their qualities and exploit new Tibetan medicines. Capillary electrophoresis （CE） is a new analysis technology. It provides advantages in terms of higher efficiency, faster separation time, less injected sample volume and more practicable operating modes. But CE has seldom been applied to the analysis of real Tibetan medicines, because the detected sensitivity of CE is lower than HPLC and the content of active constituents in Tibetan medicines is very small. So, Correlative study is developed in theory and methods about application of capillary electrophoresis in active components of Tibetan medicines.In the second part of this dissertation, the advantages of CE were made use of to separate and determinate active components in Gentianopsis paludosa （Ma）. On the basis of the previous literatures, in this dissertation, some original studies are carried out as followings:1. A novel micellar electrokinetic chromatography（MEKC） method has been developed for the simultaneous separation of 1,7-dihydroxy-3,8-dimethoxyxanthone and 1,8-dihydroxy-3,7-dimethoxyxanthone, and the proposed method has been successfully applied to determine the two xanthones in Gentianopsis paludosa （Ma）.2. A novel nonaqueous capillary electrophoresis method has been applied to separate and determinate the content of ursolic acid and oleanolic acid in Gentianopsis paludosa （Ma）This dissertation consists of three parts and four chapters.Part I Review of the development of capillary electrophoresis and Tibetan medicine and application of CE in xanthone and terpenoids compounds.Chapter 1: The principle and characteristics of CE were simply described. Study actuality of Tibetan medicines and application of CE in xanthone and terpenoids compounds were reviewed and discussed in detail.Part II Study and application of CE in Tibetan medicine Gentianopsis paludosa （Ma）.Chapter 2: A novel micellar electrokinetic chromatography（MEKC） method was developed for the simultaneous separation and determination of 1,7-dihydroxy-3,8-dimethoxyxanthone and 1,8-dihydroxy-3,7-dimethoxyxanthone in flower and stem of Tibetan medicine Gentianopsis paludosa （Ma）. The optimum conditions were: 20 mM borate - 20 mM SDS at pH 9.80, 260 nm detected wavelength and 17.5 kV applied voltage. The proposed method was not only applied for analying the two xanthone of Tibetan medicine Gentianopsis paludosa, but also an efficient technology for the preliminary study on them in other Gentianopsis paludosa plants.Chapter3: A novel nonaqueous capillary electrophoresis method was developed for the separation and determination of ursolic acid and oleanolic acid in Tibetan medicine Gentianopsis paludosa （Ma）. The optimum conditions were: 25 mM Tris -10% acetonitrile （v/v） in methanol - 1.5% acetic acid （v/v） at 214 nm UV detected wavelength and 25 kV applied voltage. It was found that flowers are the most effective parts of Gentianopsis paludosa in extracting the effective compounds.Part III Study and application of CE in Yinhuang preparations.Chapter 4: A novel, simple, sensitive, fast free solution capillary electrophoresis （FSCE） method was developed for analysis of baicalin and chlorogenic acid in Shuanghuang lian oral fluid, Yinhuang granule and Yinhuang tablet. The optimun conditions were composed of n_borate: n_NaH2PO4=1:1 （pH 8.0） at 334 nm UV detected wavelength and 15 kV applied voltage. In optimum conditions, baicalin and chlorogenic acid in three Yinhuang preparations were separated and identified within 4.3 minutes.更多还原