【作者】 白延斌；

【导师】 李伟； 瞿颂义； 郑天珍；

【作者基本信息】 兰州大学 ， 生理学， 2007， 硕士

【摘要】 围绝经期是妇女一生中重要的生理时期，妇女进入围绝经期后，卵巢功能衰退，雌激素分泌减少。由于体内雌激素水平下降，出现全身性的代谢紊乱，特别是脂质代谢，大多数妇女出现肥胖或者超重，且糖尿病、心脏病、高血压等心血管疾病的发病率明显增加。激素替代治疗(hormone replacement therapy，HRT)可有效地缓解和改善上述症状，提高绝经后妇女的生活质量，但是如果长期使用HRT，卵巢癌和子宫内膜癌的发病危险也随之增加。因此，寻找副作用小而不失雌激素作用的天然替代品已成为国内外科研工作者的热门话题。文献报道中药五味子具有植物雌激素样作用，那么五味子是否能够抑制肥胖的发生及降低心血管疾病的发病率，目前尚未见报道。本实验采用去卵巢肥胖大鼠动物模型，模拟妇女围绝经期生理状态。口服中药五味子水煎剂进行药物干预，观察五味子对肥胖大鼠体重的抑制作用以及血脂、瘦素、肿瘤坏死因子-α、丙二醛等的改变，并初步探讨其机制。选用三月龄雌性SD大鼠，体重220±2克，随机分成三组：假手术组(SHAM)，手术组(OVX)和手术+五味子组(OVX+SCB)。SHAM组和OVX组给予自来水饮用，OVX+SCB组口服五味子水煎剂，药物浓度从1％开始逐渐增加至12％后维持到实验结束。各组自由进食，饮水不限，每天定时称食量水，每10天测量一次体重。实验进行至60天，禁食12小时，股动脉放血处死大鼠，分离血清测定雌激素、血脂、肝功、血糖、胰岛素、瘦素、肿瘤坏死因子-α、白细胞介素-6、丙二醛及总抗氧化能力。所有数据都采用spss13.0统计软件处理，结果以均数±标准差((?)±s)表示，采用独立样本t检验分析，P＜0.05表示有显著性差异。实验结果如下：OVX大鼠与SHAM大鼠比较，体重和李氏指数增加，摄食量明显增加，血脂、瘦素、肿瘤坏死因子-α和丙二醛水平增高。给予五味子水煎剂后，以上指标得到明显改善。因此，本实验研究表明，中药五味子具有雌激素样作用，它能够明显抑制去卵巢肥胖大鼠摄食量和体重的增加；改善去卵巢肥胖大鼠血脂代谢的紊乱，降低血浆总胆固醇、甘油三酯和高密度脂蛋白；调节去卵巢肥胖大鼠血液中脂肪细胞因子水平，降低血清中瘦素、肿瘤坏死因子-α；增强去卵巢肥胖大鼠机体抗氧化能力，降低血中丙二醛含量。更多还原

【Abstract】 Perimenopause is an important period for women. It has well known that postmenopause women show the lower level of estrogen, thus leading to systematic metabolic disorder, especially for serum lipid disorder. Most women become obesity or overweight for this reason, as well as the incidence of cardiovascular diseases increase, such as diabetes, cardiopathy, hypertension and so on. Hormone replacement therapy (HRT) can treat these disease and prevent obesity, but increase the incidences of ovarian cancer and endometrial cancer if it was used for long time. So it has become a focus of medicine scientific researcher to find a natural product, which had few side effects. It is reported that Schizandre Chinensis Baill (SCB) has estrogen activity, but few reports are available about its effects on obesity and cardiovascular diseases in postmenopause women.To simulate metabolism of female in perimenopause and postmenopause, ovariectomized (OVX) rat model was made in this experiment. The effects of SCB’ water extract on body weight, serum lipid, leptin in ovariectomized (OVX) rat were investigated, and its mechanisms were explored. Female Sprague-Dawley rat aged three months were randomly divided into sham-operated(SHAM)group, ovariectomized(OVX)group, OVX+ SCB group according to body weight about 220±2 gramme. During the experiment, SHAM group and OVX group drank water , OVX+ SCB group drank water extract from SCB,SCB concentration was increased gradually from1% to 12%. Food and water were measured every day, all groups are free to drink and eat. Body weight were recorded every other 10 days. On the 60 day after surgery, all rats were fasted for 12 hours and then were killed. Serum levels of lipids, Hepatic function, blood glucose, Insulin, Estrogen, Leptin, Tumor Necrosis Factor-α(TNF-α), Interleukin-6(IL-6), Maleic Dialdehyde (MDA) and Total Anti-Oxidation Competence(T-AOC) were assayed. All data were statistically analyzed by independent sample t test ((?)±s) with the computer statistical pakage SPSS13.0 .difference with P<0.05 were considered significant.Our experimental results were showed as follows: compared with SHAM group, rat in OVX group displayed increased body weight, Lee’s index, food intake, Leptin, TNF-α, MDA, and serum level of lipids .While the water extract of SCB were given to the OVX+ SCB group, all of the results above have been impoved obviously. Our results indicate that SCB has estrogenic activity, which can inhibit food intake and body weight gain; improve the disorder of serum lipid metabolism, such as decrease serum levels of TC、TG、and HDL-C; regulate the adipocyte cytokine, such as decrease serum levels of leptin and TNF-α; enhance Anti-Oxidation Competence, such as decrease serum levels of MDA in ovariectomized rats.更多还原