【作者】 孙凤英；

【导师】 滕利荣；

【作者基本信息】 吉林大学 ， 微生物与生化药学， 2007， 硕士

【摘要】 鼠妇为鼠妇科动物平甲虫(Armadillidium Vulgare)的干燥全体,主要含蛋白质、蚁酸,具有解毒止痛、破血利水作用。国内曾有报道,将鼠妇用于肝癌晚期的止痛,作用持久性优于杜冷丁。本实验以鼠妇焙干体为原料,首先确定乙醇提取的最佳工艺,然后利用有机溶剂不同的极性,对鼠妇的醇提物进行初步分离,依次用石油醚、乙酸乙酯、正丁醇萃取,得到石油醚相(AVP)、乙酸乙酯相(AVE)、正丁醇相(AVB)和水相(AVW)等不同的萃取组分,最后采用传统的分离纯化方法——大孔吸附树脂和葡聚糖凝胶柱层析对有镇痛活性的萃取相进行分离和纯化,收集得到的各组分分别用小鼠扭体法致痛、热板法致痛、大鼠热刺激致痛实验确定镇痛活性。同时考察了鼠妇醇提物的抗炎和抗氧化活性,结果表明鼠妇醇提物不仅有镇痛抗炎活性,还具有较好的清除2,2-二苯基苦基苯肼自由基和羟自由基的抗氧化活性。更多还原

【Abstract】 Armadillidiam Vulgare is the whole dried body of Latreille of Armadillidiidae, and it is also called porcellio、Porcellio scaber Latreille and pillbug. It distributes widely and can be found in Hebei, Shandong, Jiangsu and Zhejiang province. The contents of reducing sugar and glycogen in Armadillidiam Vulgare vary with the growth period. Its polysaccharides contain chondritin A or C or hyaluronic acid. The lipide which Armadillidiam Vulgare contains has 10% non-saponifiable matter, 5% non-aceton solvable liquid, Fatty acids after saponification are 0.7% myristic acid, 20.6% hexadecanoic acid, 8.3% octadecanoic acid, 0.7% arachidic acid, 53.7% octadecenoic acid, 10.1% octadecadienoic acid, 2.5% jeceric acid, 3.2% other unsaturated fatty acid, and unsaponifiable matters contain 41.0% sterin and mainly is cholesterin, and maybe have formic acid. Documents recorded that Armadillidiam Vulgare has the pharmaco-factions, such as breaking blood, diuresis, detoxication and analgesia. It is reported in China that using Armadillidiam Vulgare alleviating pain of liver cancer advanced stage and its effect lasts longer than Sauteralgyl. For utilizing thoroughly traditional Chinese drug resources of our country, and finding the active components, we preliminary studied about separation and purification and pharmacological action of alcohol abstracts insolated from Armadillidium Vulgare. The results reveal pharmacological foundation of Armadillidium Vulgare, and provide rational theoretical and experimental bases for the further exploitation and utilization of Armadillidium Vulgare medicinal materials resource. The main contents are summarized as follows:The preliminary experiments were made on purification by using the dried bodies of Armadillidium Vulgare as experiment materials, pulverizating and sieving them with 20-screen cribble. Alcohol abstraction technology was optimizated by orthogonal design, and the best technology was that 5g Armadillidium Vulgare power with 20 times volume 95% alcohol recirculating extracted for 45 min. The authenticate test indicated that the technology was stabile, feasible and its reproducibility was fine.The toxicity of alcohol abstracts insolated from Armadillidium Vulgare was so low that the LD50 (mice,ig) couldn’t be determined. The maximum tolerated dose of the alcohol abstracts of Armadillidium Vulgare for Kunming species mouse was 100g crude drug/kg. The experiments demonstrated that the toxicity of the alcohol abstracts of Armadillidium Vulgare was so low that administrated by mouth was safe.A preliminary investigation was made on the analgesic action of the alcohol abstracts of Armadillidium Vulgare in the present study. The analgesic action of the said abstracts on the pseudoanalgesic response induced by chemical stimulus (mouse writhing test), on pseudo-analgesic response(mouse hot plate test and rat tail-flick test), has also been observed. Experimental results showed that the the alcohol abstracts of Armadillidium Vulgare possessed evident analgesic action. The n-butanol extracts was isolated from the alcohol abstracts of Armadillidium Vulgare by extraction with different solvents by turns, which were petroleum, acetic ether and n-butanol. Component D was attained from AB-8 and Sephadx LH-20 column chromatographic method and possessed analgesic action.A preliminary investigation was also made on the anti-inflammatory action of the alcohol abstracts of Armadillidium Vulgare, the n-butanol extracts, and the component I, II, IV and D. The anti-inflammatory effects of them had been observed using the model for xylene-induced murine ear oedema in mice.The antioxidative activities of the alcohol abstracts of Armadillidium Vulgare were examined in vitro. Results showed that AVA could scavenge hydroxyl free radical and reduce DPPH? generation, but couldn’t scavenge oxygen free radical. In recent years, there were some evidences for the clinical curative effect by modern pharmaco-research, but the vertical extent and width weren’t enough, and there wasn’t theory support for some curative effects that had verified by clinic. The pharmaco-activity investigates of Armadillidium Vulgare in the thesis provide some ideas for the further monomer purification and analgesia mechanism. They display that Armadillidium Vulgare has favourable perspective in the development of new types of analgesic which have powerful effect and no dependence.更多还原