【作者】 于志君；

【导师】 何丽娅； 成彩莲；

【作者基本信息】 武汉科技大学 ， 生理学， 2007， 硕士

【摘要】 目的:研究槲皮素抑制人胃癌MGC-803细胞淋巴转移的作用。方法:实验分组:胃癌细胞组(只加入MGC-803细胞)和槲皮素处理组(40μmol/L槲皮素)。采用免疫组化方法检测槲皮素对人胃癌MGC-803细胞中血管内皮生长因子C (vascular endothelial growth factor C, VEGF-C)及其受体血管内皮生长因子受体-3 (vascular endothelial growth factor receptor-3,VEGFR-3)蛋白阳性表达率的影响。应用半定量逆转录多聚酶链反应(Reverse transcription polymerase chain reaction,RT-PCR)的方法,检测槲皮素对VEGF-C mRNA及其受体VEGFR-3 mRNA表达水平的抑制作用。结果:免疫组化检测结果显示,胃癌细胞组中可见VEGF-C蛋白及VEGFR-3蛋白的大量表达,而槲皮素处理48 h后,VEGF-C蛋白、VEGFR-3蛋白的表达率显著下降,分别与胃癌细胞组相比两组均有显著性差异(P <0.01)。半定量RT-PCR法对人胃癌MGC-803细胞中VEGF-C mRNA和VEGFR-3 mRNA含量的检测结果显示,槲皮素处理48 h后VEGF-C mRNA和VEGFR-3 mRNA表达水平均有下调,且与胃癌细胞组相比均有显著的统计学意义(P <0.01)。结论:槲皮素具有抑制人胃癌MGC-803细胞淋巴转移的作用,其作用机制可能是通过降低VEGF-C蛋白和VEGFR-3蛋白的表达以及下调VEGF-C mRNA和VEGFR-3 mRNA的表达而得以实现的。更多还原

【Abstract】 Objects: To study the effects of quercetin on inhibition of metastasis in human gastric cancer cell line MGC-803.Methods: Cells were divided into the gastric cancer cells group(MGC-803 cells only)and the quercetin-treated group (40μmol/L quercetin) . The positive expression rate of VEGF-C and VEGFR-3 were detected by immunocytochemical staining. The expression of VEGF-C mRNA、VEGFR-3 mRNA was observed by semiquantive reverse transcription polymerase chain reaction (RT-PCR).Results: Experiments of immunity show that, a great deal of expression of VEGF-C protein and VEGF-C mRNA can be detected in the gastric cancer cells group. But after 48h since the disposal of the quercetin, the expressions of VEGF-C protein and VEGF-C mRNA decreased significantly (P <0.01).It show,in the experiment of the volume of VEGF-C mRNA and VEGFR-3 mRNA in gastric cancer cells, with the manner of VEGF-C mRNA and VEGFR-3 mRNA, that the expression of them all decreased after 48h since the disposal of the quercetin.The comparation between the two experiments have the statistics signification(P <0.01).Conclusion:Quercetin could inhibit the metastasis of MGC-803 cells. It possibly works by decreasing the protein expression of VEGF-C,VEGFR-3 and VEGF-C mRNA,VEGFR-3 mRNA expression.更多还原