血清中错位双链聚核苷酸毛细管电泳法测定研究

【作者】 赵新颖；

【导师】 谷学新；

【作者基本信息】 首都师范大学 ， 分析化学， 2007， 硕士

【摘要】 错位双链聚核苷酸是一种生物大分子，它通过调节机体自动免疫能力，激活体内RNA酶活性来降解病毒。多年来的研究结果表明：与化学药物相比，它对病毒性疾病（流行性感冒、肝炎、爱滋病、病毒性心肌炎等）的治疗效果更好。polyI：C₁₂U是经过人工合成的此类抗肿瘤药物。高效毛细管电泳凭借其高效、快速、进样量少、灵敏度高、溶剂消耗低等特点，广泛应用于大分子分离测定中。本文利用该技术考察了polyI：C₁₂U药物在动物体内代谢变化过程，得到了该药的毒性代谢情况，为此药物在临床应用提供了前期实验结果。实验突破了传统测试方式，具有快速、准确等特点。论文主要分为以下6部分内容：第一章介绍了polyI：C₁₂U的性质，综述了polyI：C₁₂U的研究现状。第二章提出了高效毛细管电泳分离测定polyI：C₁₂U的原理，制定了样品前处理方案，优化了仪器条件。第三章建立了高效毛细管电泳分离polyI：C₁₂U的模型并对此模型进行了方法学评估。第四章利用此模型对polyI：C₁₂U在Beagle犬体内代谢情况进行了监测、分析。第五章利用此模型对polyI：C₁₂U在Beagle犬体内长期毒性代谢情况进行了监测，分析。第六章总结了本论文的研究工作，指出了工作不足之处，并对下一步研究进行了设想。更多还原

【Abstract】 Double standed oligouncleotides was a kind of bio-macromolecules. The mechanism is that double standed oligouncleotides can regulate immunity of the human body, irritate RNA enzyme and decompose the virus. Compare with the chemical medicine, it has more efficient on virus and malignant tumor, such as influenza, hepatitis, AIDS （Acquired Immune Deficiency Symptoms） and virus myocarditis. polyI:C₁₂U is one of the best one of this kinds synthetic medicine. High performance capillary electrophoresis （HPCE） was used in bio-macromolecules analysis depending on its obvious advantages: high resolution, rapidly, low detection, small sample size, low solvent consumption and so on. A rapid and accurate method was developed to determine the concentration of polyI:C₁₂U in animal serum, and some useful information for the application of polyI:C₁₂U in clinical research was provided.This paper included six parts:Chapter one both introduced character and summarized the recent advance of polyI:C₁₂U.In chapter two, the principle of polyI:C₁₂U analysis by HPCE was given, the method of sample preparation and the conditions of HPCE were studied.In chapter three, the determination method of polyI:C₁₂U by HPCE was established and evulated.In chapter four, this mothed was used to determined polyI:C₁₂U in beagle metabolism.In chapter five, this mothed was used to determined polyI:C₁₂U in long-term toxicity beagle metabolism.Chapter six summarized the research work of this thesis and conceived the next objective of the study.更多还原