【作者】 张晶；

【导师】 赵俊军；

【作者基本信息】 大连医科大学 ， 病理学与病理生理学， 2007， 硕士

【摘要】 目的与背景胃癌在我国的发病率在各类肿瘤中位于第二位,死亡率居于第一位,胃癌的发生发展是多步骤多阶段多因素参与的复杂过程。Galectin-3蛋白在正常细胞和肿瘤组织中广泛表达,是碳水化合物结合蛋白之一,对β-半乳糖苷结合蛋白具有特殊亲和性,可以调节细胞生长、粘附、分化和凋亡。研究发现Galectin-3蛋白在胃肠癌、乳腺癌、甲状腺癌,前列腺癌、头颈部癌等恶性肿瘤的进展中都有影响,与胃癌进展密切相关,但报道较少,其生物学功能及在肿瘤转移中的作用还不是很清楚。P27蛋白在细胞周期调控中起重要作用,属于细胞周期蛋白依赖性激酶抑制剂,能够抑制多种Galectin-CDK复合物的激酶活性,使细胞阻滞于G1期,而抑制细胞增殖,还可促进细胞分化,诱导细胞凋亡等多种生物学作用。本文主要是探讨Galectin-3蛋白及P27蛋白在正常胃粘膜、不典型增生、早期胃癌、进展期胃癌中的表达与临床病理特征的相关性。方法采用免疫组织化学(S-P)法检测正常胃粘膜组(20例)、不典型增生组(25例)、早期胃癌组(28例)、进展期胃癌组(23例)的Galectin-3、P27蛋白表达情况。结果1、Galectin-3蛋白胃癌组织中阳性表达率为72.5%,在高、中、低分化胃癌中的阳性率分别为73.3%和71.4%。正常粘膜组Galectin-3蛋白表达阳性率( 30.0%)与不典型增生组( 44.0%)无显著差异性(p>0.05),但显著低于早期胃癌组( 64.3%)(p<0.05)和进展期胃癌组(82.6%)(p<0.01)。不典型增生组Galectin-3蛋白表达阳性率(44.0%)与早期胃癌组(64.3%)无显著差异性(p>0.05),但显著低于进展期胃癌组(82.6%)(p<0.01);早期胃癌组Galectin-3蛋白表达阳性率(64.3%)与进展期胃癌组(82.6%)无显著差异性(p>0.05)。2、正常粘膜组P27蛋白表达阳性率(85.0%)与不典型增生组(72.0%)无显著差异性(p>0.05),但显著高于早期胃癌组(42.6%)和进展期胃癌组(26.0%)(p<0.01)。不典型增生组P27蛋白表达阳性率(72.0%)显著高于与早期胃癌组(42.6%)(p<0.05)和进展期胃癌组(26.0%)(p<0.01);早期胃癌组P27蛋白表达阳性率(42.6%)与进展期胃癌组(26.0%)无显著差异性(p>0.05)。结论1、Galectin-3蛋白表达的高阳性率与胃癌发生有关,与胃癌的分化程度、TNM分期无显著相关性,与淋巴结转移有关,有可能作为发现胃癌及判断淋巴结转移的有效指标。2、P27蛋白表达的阳性率降低与胃癌发生有关,与胃癌的分化程度、TNM分期可能有关,与淋巴结转移有关,P27蛋白表达的减少可作为胃癌进展和恶性程度的一个重要指标。3、Galectin-3和P27蛋白在胃癌中的表达相反,Galectin-3蛋白和P27蛋白阳性表达在胃癌组织中没有显著相关性,但是两者同时检测有可能提高早期胃癌诊断的准确率。更多还原

【Abstract】 Objective: To investigate the expression of Galectin-3 and P27 protein and the relationship with clinicopathological features in normal gastric mucosa, atypical hyperplasia, early gastric cancer, and advanced gastric carcinoma. Galectin-3 protein expressed either in normal cells or tumor cells,it acts in process of the cell growth,adhering,differentiation and apoptosis. Some detections found that the Galectin-3 protein could infect the process of some tumous and related with gastric cancer, but the effect is not clear in metastasis of tumor. p27 protein had important action in cell cycle, it can inhibit the facts of some cyclin-CDK and inhibit the cell hyperplasia. p27 protein can promote the differentiation and apoptosis .Methods: Detecting the expression of Galectin-3, P27 protein in normal gastric mucosa, atypical hyperplasia, early gastric cancer and advanced gastric carcinoma by immunohistochemical method.Results: The positive rates of Galectin-3 protein were 30.0%, 44.0%, 64.2% and 82.6% respectively in normal gastric mucosa, atypical hyperplasia, early gastric cancer and advanced gastric carcinoma, and the positive rates of P27 protein were 85.0%, 72.0%, 42.8% and 26.0%.Conclusion: 1、the higher positive rate of Galectin-3 protein was related with the process of gastric carcinoma and metastasis of lymph node,but had no significant difference in the differentiation and TNM. It may be the significance level for diagnosing the gastric cancer and the metastasis in lymph node.2、it’s related with decreasing of p27 protein, the occurrence of gastric cancer and the metastasis in lymph node. The decreasing of p27 protein may be the important target of process of gastric cancer and thedegree of malignancy.3、The expression of Galectin-3 protein is opposite to the expression of p27 protein in gastric cancer, but had no significant difference. It may be improved the exact rate of diagnosing gastric cancer by detecting these two proteins.更多还原