【作者】 李卫东；

【导师】 刘顺英；

【作者基本信息】 东南大学 ， 内科学， 2005， 硕士

【摘要】 目的:探讨葛根素对急性胰腺炎(acute pancreatitis,AP)大鼠氧自由基的影响。方法:通过在胰胆管内逆行注射牛磺胆酸钠制成大鼠AP模型,分别检测血浆超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、淀粉酶(amylase,AMY)的变化。结果:1、从大体标本到显微镜下观察,假手术组(A组)胰腺组织均无明显改变,无腹水形成;胰腺炎组(B组)有水肿、点片状出血及黑色坏死灶,大网膜和肠壁散在皂化斑,胃肠胀气明显,有暗红色混浊腹水;镜下胰腺小叶结构模糊不清,腺泡细胞内和腺泡腔内见明显红染,有明显的炎症、水肿、出血和点状坏死,部分融合成片,内见到钙化灶和脓肿形成。葛根素组(C)组也有类似的炎症改变,有透明腹水但皂化斑数量减少,胃肠胀气不明显,镜下少见融合成片的坏死灶,无钙化灶。2、B和C两组各时点的AMY较A组明显增高。B组的SOD逐渐降低,MDA逐渐升高,和A组比较有差异,C组各时点的SOD略有下降,MDA略有升高,与A组比较无差异。结论:葛根素通过防止损伤性因子MDA的升高和保护性因子SOD的下降,对AP的治疗有一定的作用。更多还原

【Abstract】 Objective:To investigate the oxygen-derived free radicals of Rat of acute pancreatitis treated with kakonein.Methods: A retrograding injection of sodium taurocholate into pancreas and bile duct makes acute pancreatitis model of Rats’. The amylase, superoxide dismutase(SOD) depletion and malondialdehyde (MDA) level in plasm of acute pancreatitis (AP) of post-treatment with kakonein are detected.Results: A-group has no changes in pancreas specimen. all B-group have edema, edema sheet bleeding and black necrosis in pancreas specimen, saponaceous spots on greater omentum and intesting wall,obvious gastrointestinal tympanites and dark red ascitic fluid.Unclear glandular lobule texture, erythro-dye in alveolus Cells and alveolus cavity, inflammation, edema,bleeding, point-shape necrosis,some confluence and calcification Field are observed obviously under microscope.C-group have also a similar features as B-group,but the fewer number of saponaceous spots,inapparent gastrointestinal tympanites and hyaline hydroperitoneum are observed.The slight inflammation,edema,bleeding, point -shape necrosis and no calcification Field can be seen under microscop.The amylase activity in plasma of B-group and C-group is higher than that of A-group.SOD depletion and AMD progressive increases in B-group have a delimitation with A-group,whereas SOD depletion and AMD increases in C-group have no delimitation with A-group.Conclusion: Kakonein has a therapeutical effect to rats in the sodium taurocholate pancreatitis model through decreasing injury factor–MDA, and increasing protection factor– SOD.更多还原