【作者】 王志刚；

【导师】 刘明言；

【作者基本信息】 天津大学 ， 生物化工， 2006， 硕士

【摘要】 为了考察不同型式的生物气升式反应器应用于丹参不定根培养的效果,以确定最佳反应器结构。本文设计制造了一个球形气升式反应器,两个近球形气升式反应器,一个柱形气升式反应器。在这四个反应器内进行了流体力学特性及丹参不定根培养的初步研究。测定了各反应器内不同通气条件下的气含率分布,丹参不定根的最终生物量及其有效成分丹参酮ⅡA和原儿茶醛的含量。得到如下结果:反应器内气含率的研究表明,球形及近球形反应器内气体分布较均匀,在小气量下能获得较大的气含率,有利于不定根的培养。培养20天后,一号（球形）反应器增殖倍数最大,为2.37,二号（近球形）、四号（柱形）反应器内丹参不定根的增殖倍数分别为1.64倍和0.73倍,低于相同条件下摇瓶培养下的4.05倍。四个反应器内丹参不定根中丹参酮ⅡA含量分别为0.025 mg·g^-1、0.067 mg·g^-1、0.048 mg·g^-1、0.021 mg·g^-1,球形和柱形反应器培养结果略低于摇瓶培养的0.033 mg·g^-1,而近球形反应器的结果好于球形反应器、柱形反应器和摇瓶。一号（球形）、二号（近球形）、四号（柱形）反应器内的丹参不定根中原儿茶醛含量分别为0.09 mg·g^-1、0.32 mg·g^-1、0.08 mg·g^-1。球形、柱形反应器的结果略低于摇瓶培养的0.12 mg·g^-1。近球形反应器的结果好于球形反应器、柱形反应器和摇瓶的培养结果。也进行了不同培养时间的考察。纵上分析得出,近球形气升式反应器比球形反应器和柱形反应器更适合丹参不定根的培养。更多还原

【Abstract】 In order to choose the best type of airlift bioreactors for the culture of the adventitious root of S.miltiorrhzia, one sphere airlift bioreactor, two modified sphere airlift bioreactors, and one column bubble airlift bioreactor are designed in this work. The flow characteristics such as the gas holdup are investigated first to understand the air distributions in these bioreactors. The final biomass of the different bioreactor is measured and the contents of tanshinonⅡA and protocatechuic aldehyde are also measured with HPLC. The gas holdup of a bioreactor is mensurated in order. The results are as follows:It is found that the air distribution is uniformity and higher gas holdup can be obtained at low flow rate of air in sphere and modified sphere bioreactors, which is better for the culthure of the adventitious root of S.miltiorrhzia. After 20 days’culture, the multiplication of adventitious root in the first （sphere） bioreactor is 2.37, it is higher than that in the second （modified sphere） bioreactor and that in the fourth （column） bioreactor which is 1.64 and 0.73, respectively, less than that in the flask which is 4.05. The content of tanshinonⅡA of adventitious root in the four bioreactors is 0.025 mg·g^-1, 0.067 mg·g^-1, 0.048 mg·g^-1 and 0.021 mg·g^-1, respectively, and the content in the sphere bioreactor and in the column bioreactor are lower than that in the flask that is 0.033 mg·g^-1. The value in the modified sphere bioreactor is the higest. The content of protocatechuic aldehyde in the second （modified） bioreactor is 0.32 mg·g^-1. The content in the second one （modified sphere）, that in the fourth （column） bioreactor and that in the flask that is 0.09 mg·g^-1, 0.08 mg·g^-1and 0.12mg·g^-1, respectively. The culture process with different culture time is also investigated. It is reasonable that the modified sphere airlift bioreactor is the best one for the culture of the adventitious root of S.miltiorrhzia from the experimental investigations of this thesis.更多还原