【作者】 赵勇；

【导师】 王宸；

【作者基本信息】 东南大学 ， 外科学， 2006， 硕士

【摘要】 试验一白介素-1受体拮抗剂对双后肢大鼠椎间盘细胞凋亡的影响目的:建立双后肢大鼠模型,探讨白介素-1受体拮抗剂(IL-1Ra)对双后肢大鼠椎间盘细胞凋亡的影响。方法:建立双后肢大鼠模型54只,随机分为试验组、空白对照组和阴性对照组,每组18只,试验组从造模当天即予腹腔注射IL-1Ra(50ng/kg体重),隔天重复注射至处死,空白对照组共18只不予任何处理,阴性对照组共18只,隔天予腹腔注射等量生理盐水。于造模后1、3、6个月每组分别处死6只大鼠,完整取出L4∕5椎间盘,固定、切片,使用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法(TUNEL法)检测各组双后肢大鼠椎间盘细胞的凋亡情况,并使用透射电镜观察凋亡细胞的超微结构和纤维环的排列情况。取L5∕6椎间盘使用流式细胞仪检测各组双后肢大鼠椎间盘细胞的凋亡率。结果:术后第一个月试验组、空白对照组及阴性对照组大鼠椎间盘细胞的凋亡数目相近,无显著性差异(P >0.05),第三个月试验组与空白对照组及阴性对照组相比凋亡细胞明显减少,有显著性差异(P <0.05),第六个月试验组与空白对照组及阴性对照组相比凋亡细胞减少更为明显,有非常显著性差异(P <0.01)。结论:IL-1Ra对双后肢大鼠椎间盘的细胞凋亡有明显的抑制作用。更多还原

【Abstract】 PartⅠThe effects of the IL-1Ra on the apoptosis of the intervertebral disc cell of the bipedal ratObjective:To explore the effects of the interleukin?1 receptor antagonist protein (rhIL-1Ra)on the cell apoptosis of the bipedal rats’ intervertebral disc.Methods:To establish a degenerative model a total of 54 bipedal rats.The 54 rats were randomly divided into three groups:experimental group,blank-control group and negative-control group,each group has 18 rats.The experimental group rats were intraperitoneal injected with IL-1Ra(50 ng per kilogram of body weight) from the day of operation and repeated it on alternate days until they were killed,and the negative-control in which 0.9%NS of the same dosage as IL-1Ra were injected. The blank-control group without treatment.Six rats were killed from every group at one、three、six months post-operation respectively.The tissue of L4/5 discs were fixed and sliced.The apoptosis cells of the intervertebral disc were assayed with terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL) and the ultrastructure were examined by transmission electron microscopy,and the tissue of the L5/6 discs were assayed with flowcytometry.Results:The statistic analysis of three groups had no significant difference at a month post-operation(P >0.05). The apoptosis cells of the experimental group had obviously decreased than blank-control group and negative-control group at three months post-operation, the statistic analysis had significant difference(P <0.05). The statistic analysis of three groups had very significant difference at six months post-operation(P <0.01)。There is no significant different between blank-control group and negative-con trol group at 3,6month post-operation(P >0.05).Conclusions:IL-1Ra protein can inhibit the apoptosis of the intervertebral disc cell of the bipedal rat.更多还原