【作者】 毕玉海；

【导师】 丁壮；

【作者基本信息】 吉林大学 ， 预防兽医学， 2006， 硕士

【摘要】 鹅细小病毒病(俗称小鹅瘟)与鹅副粘病毒病是严重危害水禽养殖业的两大重要病毒性疾病,在临床症状上十分相似,不易区分,给水禽养殖业带来了严重的经济损失。为进一步研究GPV和MDPV感染谱、病毒分子致病机理、为下一步更深入地研究NDV跨种间传播的分子生物学机制奠定基础,及有效预防控制这两种疾病的发生和流行,本研究以本实验室分离、保存的GPMV NA-1株、GPV GD-01株为研究对象,与国内外相关毒株分析比较了GPMV NA-1株主要免疫原性蛋白F基因、GPV GD-01株主要免疫原性蛋白VP3基因的遗传变异情况。并对这两种主要免疫原性基因进行了表达研究。遗传变异研究显示:GPMV NA-1株的F基因全长1 662 bp,编码553个氨基酸,与已发表的5株GPMV毒株核苷酸、氨基酸同源性分别平均为97.76%、98.0%,与国家标准毒株F48E9、LaSota传统弱毒株的核苷酸、氨基酸同源性分别为87.4%、92.2%,84.8%、89.0%,与其他鸡源NDV毒株核苷酸、氨基酸的同源性也都在84.7%以上;由NA-1株的F基因推导的氨基酸序列,在其裂解位点的氨基酸组成具有NDV中强毒株的标志,即112R-R-Q-K-R-F117。同时具备VII型NDV的典型特征,即101位的K和121位的V两个特征性氨基酸,结合F基因进化树分析确定,NA-1株与近年来流行的GPMV毒株同属F基因VII型、即APMV-1;抗原表位分析比较显示,不同宿主来源的NDV F蛋白抗原表位不尽相同,不同地域GPMV毒株的F蛋白抗原表位不尽相同,同时发现猪源NDV SP13毒株与LaSota株、鸭源NDV FM1/03株、阿根廷侯鸟分离株88T.00株、鸡源NDV ZJ/2000株抗原表位基本相同。GPV GD-01株VP3基因全长1 605 bp,编码534个氨基酸。GPV GD-01株与其他GPV、MDPV毒株的VP3基因核苷酸序列同源性分别平均为96.15%、89.16%,与HG5/82、YG株同源性最高分别达到99.8%。与其他GPV、MDPV毒株的氨基酸更多还原

【Abstract】 Derzsy’s disease is a acute or subacute septic infectious disease of gosling with a high mortality caused by Goose Parvovirus(GPV). Muscovy Duck parvorirus(MDPV)is similar to GPV in many ways,such as morphology,physical and chemical character,biochemistry,cultural character,immunological character and genomic structure,though there are many differences in pathogenicity. Not only can GPV infect goslings,but also Muscovy ducklings,but MDPV infect Muscovy ducklings exclusively. In order to further study the infection spectrum differences of GPV and MDPV,and the method for prevention and control the Derzsy’s disease,the genetic variation studying of epidemic strains of GPV is very important.Goose paramyxovirus disease with a high morbidity and mortality,caused by Goose paramyxovirus(GPMV),a member of Paramyxoviridae. The digestive system is the primal character of clinical symptom and pathological changes. GPMV can infect all varieties and all ages of geese. The infection spectrum of Newcastle disease virus(NDV) has been extending endlessly. From NDV infected geese in 1 997,the low virulent strain of NDV was also isolated in Muscovy ducks and swine from 2 000 to 2 005. So the study of the genetic variation of GPMV,an antigenic variant of NDV,have a important meaning to further study the molecular biology mechanism of species-breaking spread of NDV.According to the nucleotide sequence of GPMV in GenBank,a pairs of primers更多还原