【作者】 李武客；

【导师】 李松军；

【作者基本信息】 华中师范大学 ， 有机化学， 2006， 硕士

【摘要】 微米级聚合物微球由于具有比表面积大、单分散性好、易于制备及功能化和生物体相容性好等优点，已成为近年来研究最为热门的课题之一。应用聚合物微球不仅可以部分替代天然生物材料，而且能极大地扩展天然材料的适用范围，从而在生物医学、材料科学、信息科学、食品、化工及生物过程和环境监控等过程显示出广阔的应用潜力。 本文在功能高分子微球的合成与功能化、载体微球与生物大分子的相互作用及经典吸附理论的改进等方面进行了一系列的探索性工作。在载体微球合成中，研究采用了分散聚合法一步合成表面荷有磺酸基及醛基两种微米级单分散具有表面反应活性聚合物微球。与生物大分子的相互作用研究中，本文以牛血清蛋白等生物大分子为靶分子，以功能微球为载体，系统研究了载体与生物大分子的相互作用机制。研究表明，生物大分子与功能高分子微球之间的相互作用远比经典理论体系如Langmuir、Freundlich及Temkin等更为复杂，经典理论的适用应具有明显的限制。为之，本文对经典理论进行了发展和延伸，获得了评价其相互作用机制的更好方法。此外，研究还表明吸附固定化过程对生物靶分子如酶等的活性、稳定性及构像等能构成重要的影响。酶的研究表明固定化不仅提高了酶的耐热及储存稳定性，而且能增加其抗酸碱性能，从而为其长时间连续的应用创造了有利条件。更多还原

【Abstract】 Micron-sized polymer microspheres, due to its advantages of high specific-surface area, monodispersity as well as facile preparation and functionalization, have been one of focuses in the recent years. The application of polymer microspheres can not only substitute, in part, natural biomaterial but also expand its applicable range largely, thereby presenting a wide prospective for various fields such as biomedicine, materials, information, food, chemical engineer and biological fields etc.In the present dissertation, a series of tentative works involving the synthesis of micron-sized polymer microspheres and their functionalization, the interaction between the synthesized support and bio-macromolecules as well as the modification of classic theories were presented. For the synthesis, one-stepwise dispersion-polymerization was employed. Two micron-sized monodispersity polymers with bioactivity surfacially was prepared based on the participated monomer which contains active aldehyde and sulfonic groups. With bovine serum albumin as target molecule and the synthesized microspheres as support, the interaction mechanism of support-biomacromolecule was studied. Related information indicated that the interaction mechanism is much more complicated in comparison to classic theoretical systems including idealized Langmuir model and practical Freundlich or Temkin theories. To interpret the practical process better, the classic theories were developed and which ultimately presents a more logical result. Further information also reveals that the adsorption and immobilization of biomacromolecule such as enzyme have significant effect on the activity, stability and conformation etc. The immobilization improves not only the performance of thermo-resistance and the depositing stability, but also increases the capability of anti-acids or bases, which thus pave the way for longtime and consecutive uses.更多还原