【作者】 常峰；

【导师】 王忠彦；

【作者基本信息】 四川大学 ， 微生物学， 2006， 硕士

【摘要】 从四川某浓香型大曲酒酿造企业窖池里的黄水中，通过厌氧分离技术、纸片法初筛、三角瓶扩大培养、杯碟法复筛，得到一株代谢产物对革兰氏阳性致病菌、革兰氏阴性致病菌及部分真菌和酵母菌具有抑制作用的乳酸菌，菌株编号CF10。经形态观察、生理生化及分子生物学实验，鉴定该菌为布氏乳杆菌（Lactobacillus buchneri）。在排除酸性末端产物和过氧化氢的干扰作用后，CF10发酵上清液仍具有明显的广谱抑菌作用，故将这一抑菌活性成分纳入类细菌素的范畴。 在类细菌素高产菌株的选育过程中，采用紫外线（UV）与硫酸二乙酯（DES）多轮复合诱变，类细菌素高产菌发酵液相对效价较未诱变前提高了258％且具有良好的传代稳定性。 通过单因素分析和正交试验法，对目的菌CF10的培养基组成和发酵条件进行了探讨，确定了CF10的最佳培养基配方为：以2％麦芽糖为碳源，1％大豆蛋白胨加0.5％酵母粉为氮源，有微量K⁺、Mg²⁺、NH₄⁺、Mn²⁺等金属离子存在。最适发酵条件为：培养温度37℃，起始更多还原

【Abstract】 A novel bacteriocin-like substance producing strain was screened from yellow-liquor samples by anaerobic isolation and scrip method and cylinder-dish method and was named CF10. The supernatant of CF10 culture have strong bacteriostasis activity against the gram-positive pathogenic bacteria and the gram-negative pathogenic bacteria and some fungi and microzyme. The strain was identified as Lactobacillus buchneri by morphologic characteristics and physiological-biochemical testing and molecular-biological testing. Excluded the interference of organic acids and hydrogen peroxide, strong bacteriostasis activity was also observed, we belong it to bacteriocin-like substance.In the process of mutation breeding bacteriocin-like substance high-producing strains, CF10 was treated with multiple times compound mutation by ultraviolet (UV) radiation and diethyl sulphate (DES), the relative potency of supernatant had increased 258% and it have stable inheritance.更多还原