【作者】 李文娟；

【导师】 侯太平；

【作者基本信息】 四川大学 ， 农药学， 2006， 硕士

【摘要】 本文以高原药用植物瑞香狼毒(Stellera chamaejasme L．)为材料，金黄色葡萄球菌(Staphylococcus．aureas)、大肠杆菌(Escherichia．coli)、白色念珠菌(Candida albicans)、红色毛癣菌(Trichophyton．purpureatum)、石膏样毛癣菌(Trichophyton．gypsum)、石膏样小孢子菌(Microsporum．gypsum)、絮状表皮癣菌(Epidermophyton．floccosum)为供试菌。研究了瑞香狼毒的活性成分对供试菌的抑制活性，并对其作用机制进行了初步研究。采用药敏纸片法研究了瑞香狼毒不同溶剂提取物、萃取物对供试菌的抑菌活性，以药基法测定了乙酸乙酯萃取物对供试菌的最低抑菌浓度MIC和最低杀菌浓度MBC和MFC。并且进一步选定金黄色葡萄球菌和石膏样毛癣菌为代表，研究了瑞香狼毒对细菌和真菌生长的抑制、对其生长期的影响、对细胞膜的影响以及体内细胞壁酶和蛋白酶活性的影响，初步推测瑞香狼毒的抑菌机制。实验结果表明：瑞香狼毒的95％乙醇提取物抑菌效果较好，进一步系统溶剂萃取后，得到的乙酸乙酯萃取物和90％甲醇萃取物的抑菌活性最强，乙酸乙酯萃取物对金黄色葡萄球菌和石膏样小孢子菌的抑菌圈直径可达12.02mm和9.04mm，90％甲醇萃取物对红色毛癣菌的抑菌圈直径可达7.73mm，故推断抑菌活性成分主要为中偏强极性物质。定量测定MIC、MBC、MFC，表明瑞香狼毒对大肠杆菌和白色念球菌是抑制作用，对其他供试菌为杀灭作用，尤其对真菌组中的石膏样毛癣菌的最低抑菌浓度和最低杀菌浓度为0.31mg／ml，对细菌组中的金黄色葡萄球菌的最低抑菌浓度和最低杀菌浓度为0.63mg／ml，效果最好。瑞更多还原

【Abstract】 The antibiotic activities and mechanism of the extracts of the root of the Stellera chamaejasme L. were tested against S.aureas, E.coli, Candida albicans, T.purpureatum, T.gypsum, M.gypsum and E.floccosum. The herbal was extracted by acetone, 95% ethanol, methyl alcohol, water, acidic water and alkaline water. The extract of the 95% ethanol was again extracted by petroleum ether, ethyl acetate and 90% methyl alcohol. The extracts were tested for the antibiotic activities by Neo-Sensitab disk-diffusion method. MIC MBC and MFC also were tested by test tube method. Using T.gypsum and S.aureas as representative fungi and bacteria species, we studied the amount of the growth, shape, growth curves, cell membrane and some enzymes activity.The results showed that the active component existed in the ethyl acetate extract(EAE) and 90% methyl alcohol extract(MAE). The maximum mean diameter of antibiotic circle of ethyl acetate extract in 33.33g/L against S.aureas is 12.02mm and against M.gypsum is 9.04mm. The maximum mean diameter of antibiotic circle of 90% methyl alcohol extract in 33.33g/L against T.purpureatum is 7.73mm. EAE inhibited the Candida albicans and E.coli, but killed the others. The MIC and MFC of T.gypsum were 0.31mg/ml. The MIC and MBC of S.aureas were 0.63mg/ml. The amount of the growth were更多还原