【作者】 张露洁；

【导师】 傅华龙；

【作者基本信息】 四川大学 ， 植物学， 2006， 硕士

【摘要】 自然界中许多重金属都是是植物的非必需元素，对植物生长和发育具有显著的毒性，当细胞内的重金属离子超越了保护酶系统的防御能力后，在细胞中游离的重金属离子会通过不同的途径干扰和破坏细胞的正常代谢，导致植物生长异常直至死亡。重金属在植物根、茎、叶及籽粒中的大量累积，不仅严重地影响植物的生长和发育，而且会进入食物链，危及人类的健康。自80年代以来，国际社会对我国出口的中成药中重金属和／或砷含量超标问题反应强烈，使之成为国际医药市场的热点话题，严重损害了中药的形象，造成了极坏的影响，并严重影响了中成药的出口创汇，在经济上造成了极大的损失。 研究重金属对植物生长的影响对于生产绿色食品和中药、治理环境污染、保护生态环境和发展国民经济有重要意义。本研究以Cd²⁺、Hg²⁺、Cr⁶⁺为影响因素，研究不同浓度下三者单独和交互作用对组培盾叶薯蓣苗部分生理指标的影响，从而为重金属对植物的毒理研究提供一定的实验数据。 本论文主要分为两个部分： 第一部分研究了Cd²⁺、Hg²⁺、Cr⁶⁺复合作用对组培盾叶薯蓣叶绿素含量的影响。结果显示，组培盾叶薯蓣叶绿素含量在Cd²⁺、Hg²⁺、Cr⁶⁺各处理浓度条件下均不同程度地受到抑制作用，其抑制效应随重金属浓度的增加而增强。即使Cd²⁺、Hg²⁺、Cr⁶⁺的浓度符合环境质量标准，这三种重金属离子仍然会对组培薯蓣叶绿素的含量造成影响。Cd²⁺、Hg²⁺、Cr⁶⁺对组培薯蓣叶绿素a、b的含量的影响的大小顺序为：Cd²⁺＞Cr⁶⁺＞Hg²⁺＞Cd²⁺、Hg²⁺、Cr²⁺互作＞Hg²⁺、Cr⁶⁺互作＞Cd²⁺、Cr⁶⁺互作＞Cd²⁺、Hg²⁺互作。 第二部分研究了盾叶薯蓣的部分生理指标在Cd²⁺、Hg²⁺、Cr⁶⁺胁迫下的变化更多还原

【Abstract】 As unnecessary elements of plants, heavy metals （Hg、 Cd、 Pb、 Cr） have serious toxicity to the growth and maturity of plants. When the toxicity in heavy metals make the protective enzyme system lose defense capability, the nomadic ions of heavy metals in the cells will molest and destroy the cells’ normal homergy in various ways, which directly results in abnormal growth of plants until their death. The huge amassment of heavy metals in roots, stems, leaves and seeds, not only seriously affects the growth and maturity of plants, but also could enter the food chains and be dangerous to the health of human-being. From 1980s, the international society have strong response to the heavy metals and As excess content in the prepared Chinese medicine exported abroad, and make it a heated discussion in the global prepared Chinese medicine market that seriously damages the good faith of prepared Chinese medicine and make great loss to economy because of less foreign currency.It’s meaningful for the production of green food and prepared Chinese medicine, cure of environmental pollution, protection of ecological environment and the development of national economy to research the impact of heavy metals to the growth of plants. Regarded Cd²⁺、 Hg²⁺、 Cr⁶⁺ as influential factors, this paper do research on the above three factors’ independent impact and interaction under different strengths upon partial physiologic index of the tissue cultured dioscorea zingiberensis and provide certain experimental data for heavy metals’ impact to更多还原