【作者】 龚晓霞；

【导师】 侯太平；

【作者基本信息】 四川大学 ， 植物学， 2006， 硕士

【摘要】 瑞香狼毒（Stellera chamaejasme L.），瑞香科狼毒属植物，是青藏高原草地上分布的一种重要有毒植物，本文针对其在抗菌方面的功效进行了系统的研究。以人体病原细菌金黄色葡萄球菌（S.aureas）、大肠杆菌（E. coli），深部真菌白色念珠菌（Candida albicans），浅部真菌石膏样毛癣菌（T.gypsum）、石膏样小孢子菌（M.gypsum）、絮状表皮癣菌（E.floccosum）、红色毛癣菌（T.purpureatum）为供试菌，采用无水乙醇为溶剂对瑞香狼毒根粉进行了总提，再以不同极性溶剂对狼毒总提取物进行了分级萃取，所得的萃取物的生物活性测试结果表明：瑞香狼毒对人体常见病原细菌和真菌有抑制作用，乙酸乙酯萃取物在33.33g／L浓度下对细菌组的抑菌圈直径最高可达12.02mm，对真菌组最高可达9.04mm。 在采用不同溶剂对总提取物萃取的基础上，继续用薄板层析、分段正相柱层析等分离方法并结合以石膏样毛癣菌为代表菌的生物活性的跟踪试验，从乙酸乙酯萃取物中分离纯化抑菌活性成分。结果从中获得了一个高活性高纯度样品，样品为浅黄色油状物。该化合物对石膏样毛癣菌的最低抑菌浓度（MIC）为9.8mg·L^-1。通过高分辨质谱并结合波谱数据推测其可能结构图如下所示。更多还原

【Abstract】 Stellera chamaejasme L. belongs to Stellera L. Thymelaeaceae, heavily toxic. This thesis made a systematic research about its application on anti-fungal. The antifungal activity of Stellera chamaejasme L. was tested in vitro against E.coli, S.aureas, Candida albicans, T.purpureatum, T.gypsum, M.gypsum, and E.floccosum respectively. In this paper, the author extracted from the root of Stellera chamaijasme L. with anhydrous ethanol, and made gradient extraction from it with different solvents. The results showed that Stellera chamejasme L. has significant antifungal activity against test pathogens. The maximum mean diameter of antibiotic circle of ethyl acetate extract in 33.33g/L against bacteria is 12.02mm and against epiphyte is 9.04mm.A high purity and high active ingredient was isolated by by a series of methods such as TLC, normal phase chromatography, and the minimum inhibitory concentration （MIC） was 9.8 mg· L^-1. It was light yellow oil, showing fluorescence in UV. The structure was determined by the spectrum data. This work was the basis of using the root of Stellera chamaejasme L. to produce new natural antifungal medicine.更多还原