【作者】 魏莉；

【导师】 袁萍；

【作者基本信息】 四川大学 ， 流行病与卫生统计学， 2006， 硕士

【摘要】 [目的] 了解贵州省贵定县哮喘儿童居室内燃煤型空气污染物（PM₄、SO₂、CO）的暴露水平和哮喘儿童中β₂肾上腺素能受体（β₂-AR）基因第16、27位点以及血管紧张素转移酶（ACE）基因的分布频率，进而揭示燃煤型室内空气污染物（PM₄、SO₂、CO）和哮喘易感基因的交互作用与儿童哮喘发病风险的关系。 [方法] 本次研究采用单纯病例研究的流行病学方法。在贵州省贵定县收集研究对象的基本信息和血样，通过PCR方法检测每个研究对象的β₂-AR基因第16、27位点多态性和ACE基因多态性，同时分别检测当地县城和乡村家庭居室内空气中PM₄、SO₂、CO的浓度，计算三种哮喘易感基因的基因型分布频率和等位基因频率，并判断燃煤型室内空气污染物（PM₄、SO₂、CO）与三种哮喘易感基因多态性在儿童哮喘的发生中是否存在相乘模型的交互作用。 [结果] 县城居民家庭室内的PM₄、SO₂和CO浓度均未超标，乡村居民家庭室内的SO₂浓度超标率达到61％，PM₄浓度超标率达到67％，CO浓度未超标。研究对象β₂-AR基因16位点Arg／Arg纯合子、Gly／Gly纯合子、Arg／Gly杂合子的频率分别为13.9％、13.4％和72.7％，27位点Gln／Gln纯合子、Glu／Glu纯合子、Gln／Glu杂合子的频率分别为13.9％、4.3％和81.8％，ACE基因Ⅱ、DD和ID基因型的频率分别为49.3％、20.0％和30.7％。另外，未考虑其它因素的更多还原

【Abstract】 [Objectives] To take the measurements for indoor air pollutants from burning coal in Gui Ding, Gui Zhou province, including PM₄、SO₂、CO. Measurements were taken in asthmatic children’s house. To detect 16, 27 locus of β₂-AR and ACE genetic polymorphisms. So as to find out the linkage between childhood asthma and interaction of indoor air pollutants from burning coal and genetic polymorphisms susceptible to asthma.[Methods] Basic informations and blood samples of asthmatic children were collected. PCR was used to detect 16, 27 locus of β₂-AR and ACE genetic polymorphisms. Measurements for PM₄、SO₂、CO were taken in county and country respectively. Genotype frequencies of three genetic polymorphisms were calculated. Using the epidemiologic method of case-only study, the interaction of indoor air pollutants from burning coal and genetic polymorphisms susceptible to asthma was analyzed.[Results] PM₄, SO₂ and CO concentrations of county are within common standards. PM₄, SO₂ concentrations of country are in excess of common standards for 67% and 61% respectively while CO concentration is within guideline. The frequencies of更多还原