【作者】 王琪；

【导师】 林苹；

【作者基本信息】 四川大学 ， 肿瘤学， 2006， 硕士

【摘要】 研究背景及目的：肿瘤是一种严重危害人类生命的疾病，而缺乏专职抗原提呈细胞(APC)对肿瘤抗原的有效提呈，导致初始型T细胞与肿瘤细胞的相互作用耐受或无能是造成肿瘤患者体内阻碍T细胞介导的抗肿瘤免疫有效激活的主要原因之一。树突状细胞(DC)是最具潜能的专职APC，能激活初始型T细胞产生免疫应答，DC介导的抗肿瘤免疫治疗是肿瘤免疫治疗的重要组成部分。DC的功能依赖于DC的分化成熟状态，成熟DC才能有效激活肿瘤免疫应答，而未成熟DC会诱导免疫耐受。因此，研究影响DC分化成熟的因素，从而设法增加DC的成熟度，启动有效的抗肿瘤免疫，成为DC介导的抗肿瘤免疫治疗的关键问题。 LIGHT(for homologous to lymphotoxins，exhibits inducible expression，and competes with HSV glycoprotein D for HVEM，a receptor expressed by T lymphocytes)是1998年新发现的TNF超家族的成员，LIGHT的两个受体HVEM和LTβR在DC上有表达。LIGHT可通过激活NF-κB信号通路上调DC表面共刺激分子表达并诱导其成熟；共刺激T细胞，加强抗原特异性的细胞毒性T淋巴细胞(CTL)反应；还可诱导肿瘤细胞凋更多还原

【Abstract】 Background & Objective: Carcinoma is one of the main causes which lead to death. As the absence of tumor-antigen cross-presentation by professional antigen-presenting cells (APC), the interaction of naive T cells with neoplastic cells may lead to either tolerance or anergy. This is one of the reasons that block the T cell-mediated antitumor immunity. Dendritic cells (DC) are the most potent APC and stimulators of T cell activation. DC-mediated antitumor immunetherapy is an important part of antitumor therapy. It is mature DC but not immuature DC which can stimulate naive T cell. Cytokine can regulate the maturation of DC to enhance DCs ability in presenting antigen, and achieve the objective of activating the immune system. Therefore, investigating the factors that contribute to DC differentiation and maturation is necessary for the development of DC-mediated antitumor immunetherapy.LIGHT (for homologous to lymphotoxins, exhibits inducible expression , and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), a recently identified cytokine, can regulate the molecular更多还原