【作者】 赵亮；

【导师】 李兰生；

【作者基本信息】 中国海洋大学 ， 环境工程， 2006， 硕士

【摘要】 本文研究了光合细菌及固定化光合细菌在含染料废水处理中的应用。(1)以海水底泥为原料,将光合细菌筛选出来,分离纯化得到光合细菌纯种,并以光合细菌纯种为基础进行扩大化培养,探讨适宜的培养条件,包括合适的pH、温度及盐度对光合细菌的影响。(2)将扩大化培养得到的光合细菌用来处理模拟染料废水,并探讨了光合细菌对染料脱色的适宜条件,包括外加碳源的选择、pH、温度、实验培养基的碳氮配比、细菌接种量以及染料浓度和染料结构对脱色反应的影响。结果表明所用菌种对五种染料都有很好的脱色效果。温度对各类染料影响不同,但五种染料都在30-35℃时达到最佳脱色效果。菌种适应的pH值范围广,驯化后活性增强。在100mg/L的浓度下,染料X-3B对细菌生长没有明显的抑制作用。而以葡萄糖为外加碳源、由酵母膏提供氮源时,随着葡萄糖浓度的增大,脱色率反而下降;酵母膏的浓度对脱色效果影响很大,它在脱色过程中起到氮平衡的作用;当葡萄糖和酵母膏的浓度分别为0.125%和0.3%时,X-3B的脱色效果最佳。所选实验条件下,染料浓度在500mg/L以下X-3B脱色效果很好,对菌种没有明显的抑制作用;染料浓度在500-1000 mg/L之间变化时,虽然对菌种有抑制作用,染料最终仍能完全脱色。温度在30℃,pH在3-10范围内时对染料有较好的脱色效果。(3)采用海藻酸钠对光合细菌进行包埋,尝试利用添加纳米材料和壳聚糖的途径提高固定化细胞对染料的处理效果,并确定了添加纳米材料的固定化细胞的最佳固定化条件,对振荡、钙化时间、固定化细胞的重复使用对脱色效果的影响也做了研究。结果表明添加纳米材料的固定化光合细菌对X-3B的脱色效果较好。纳米材料的添加改变了小球的传质效率,从而加快了降解速率。而加入壳聚糖的固定化光合细菌在厌氧条件下对X-3B的脱色效果良好,染料浓度在30～300mg/L之间变化,厌氧降解动力学方程均符合一级动力学方程,不同浓度染料的降解速率与浓度之间的关系符合Michaelis-Menten方程,反应动力学参数Km=374.96 mg/L,更多还原

【Abstract】 Applicaton of photosynthetic bacteria on the treatment of simulated dye wastewater was investigated in this dissertation.Firstly PSB was acclimated from sea mud Under selective culture conditions . pure culture of PSB was isolated by means of agar dilution.Secondly, Effect of temperature,pH and salinity on PSB were investigated on the base of pure culture .Then the effects of ocean photosynthetic bacteria on decolourization of five dyes under different conditions such as the choice of the carban source, pH, temperature, C:N of the culture, dye concentration, the dye structure were studied. Results show that ocean photosynthetic bacteria could tolerate high salinities and five different dyes were decolorized by it. The effect of temperature on the dye with different structure is different. The decolourization rate was optimal at temperature 30-35℃, X-3B was degraded well under the condition of PH ranged from 3 to 10. The activity of tamed bacteria was better. X-3B did not inhibit cell growth when its concentration≤100 mg/L.When the carbon source was glucose and nitrogen source was yeast extract, the decolourization rate reduced with the concentration of glucose increasing ; the concentration of the yeast extract affected the decolourizaion obviously and its major role in the medium is a N-balancing compound ; the decolourization rate is optimal when the concentratin of glucose and yeast extract was 0.125 % and 0.3 % respectively . Under the optimal condtion , X-3B was decolourized well when the dye concentration≤500 mg/L and it did not inhibit cell growth; with 500mg/L<dye concentration≤1000 mg/L, X-3B could be decolurized completely though it inhibited cell growth .Finally, PSB was immobilized and applied to the treatments of simulated dye更多还原