【作者】 魏琳；

【导师】 陈由强；

【作者基本信息】 福建师范大学 ， 植物学， 2006， 硕士

【摘要】 以复苏植物——卷柏(Selaginella tamariscina(Beauv．)Spring)为研究对象，运用植物生理生态学理论和方法，研究其在5％、10％、15％三种聚乙二醇(polyethylene glycol，PEG)浓度模拟干旱胁迫处理下的生理变化情况。进而应用蛋白质组学方法探究其在干旱胁迫条件下蛋白质组的变化，旨在探索卷柏抗旱逆境生理与所产生的蛋白质之间的关系，为从分子水平揭示卷柏抗旱机制奠定基础。同时也对双向聚丙烯酰胺凝胶电泳系统进行了优化。 本论文测定了卷柏叶片在不同浓度PEG干旱胁迫处理下的脯氨酸含量变化以及叶片质膜透性、超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性随PEG浓度和时间进程的变化。通过多种条件的组合与优化，建立了一整套从卷柏叶片中提取总蛋白质的方法，同时以双向电泳为研究手段，以银染分析出对照与干旱处理下卷柏的差异蛋白质，并采用考马斯亮兰染色法富集差异点，总共分离并检测到18个蛋白质差异点样品，根据肽质量指纹图谱检测结果及Mascot检索数据库初步鉴定出其中的7个蛋白质点，其它11个为未知的新蛋白。并进一步探讨了所鉴定出的7个蛋白质在干旱胁迫中的生理功能。更多还原

【Abstract】 We chose Selaginella tamariscina (Beauv.) Spring which is a kind of resurrection plants as our experiment sample. With 5%、 10%、 15% PEG to imitate drought stress, we researched the physiological change of S. tamariscina (Beauv.) Spring with the theory of plant Physiological ecology. And then we made use of the functional proteomics to explore the varieties protein of S. tamariscina (Beauv.) Spring which was under the PEG stress. The purpose of our experiment was that discussing the relation of drought-resistance and plant protein under PEG stress, and atempted to reveal mechanism of drought-resistance of S.tamariscina (Beauv.)Spring in molecular level. At the same time, we controled and optimized the experimental conditions of the two-dimensional gel electrophoresis system.The research determined the content variety of proline in leaves of S. tamariscina (Beauv.) Spring with deal time under different PEG concentration stresses, as well as the permeability of plasma membrane, SOD activity and POD activity were measured. By combination and optimization of various conditions, a sample preparation method from leaves of S.tamariscina (Beauv.) Spring was established. And then we founded a set of method of two-dimensional gel electrophoresis, the discrepancy spots which were in the comparison and drought stress, were analyzed by silver staining. Whereafter we accumulated the discrepancy spots by the coomassie brilliant blue staining, and then 18 spots were obtained, mapping peptide mass fingerprinting of protein spots by MALDI-TOF-MS, 11 spots were identified with new protein, and 7 spots were identified with known proteins from protein database using Mascot, physiological functions of 7 proteins were analysed at the course of S.tamariscina (Beauv.) Spring drought stress.更多还原