【作者】 段链；

【导师】 吴春福；

【作者基本信息】 沈阳药科大学 ， 药理学， 2004， 硕士

【摘要】 本论文应用碱性单细胞凝胶电泳技术研究了临床常用的24种清热解毒药及其药对对小鼠外周血淋巴细胞DNA的损伤作用，为中药的遗传毒理学研究提供佐证，并对临床合理用药作以指导和提示。 1．在比较单细胞凝胶电泳全血法和分离淋巴细胞法的研究中发现，上述两种方法均能检测到两种损伤剂乙醇(1g／kg，2g／kg，i.p.)、重铬酸钾(10 mg／kg，20 mg／kg，i.p.)所导致的小鼠外周血淋巴细胞DNA损伤，且作用趋势相同。因此，采用全血进行彗星试验更为简便。 2．在对单味清热解毒药对细胞DNA的损伤程度的研究中，本实验选用1/3临床等效量，临床等效量，3倍临床等效量，分别给药1天、3天、7天，对小鼠外周血淋巴细胞DNA进行损伤性研究。结果表明： (1) 金银花、马齿苋三个剂量、三个时间点对小鼠外周血淋巴细胞DNA均无损伤作用； (2) 苏铁蕨(贯众)、板蓝根三个剂量、三个时间点对小鼠外周血淋巴细胞DNA均有损伤作用，其中苏铁蕨(贯众)具有剂量依赖关系； (3) 忍冬藤三个剂量给药1天，射干三个剂量给药3天、7天时对小鼠外周血淋巴细胞DNA有损伤，且损伤程度呈剂量依赖趋势。 (4) 大青叶、野菊花、忍冬藤、穿心莲、蒲公英、白头翁、射干给药1天；五指莲(重楼)、半枝莲、苏铁蕨(贯众)、鱼腥草、败酱草、北豆根、射干给药3天； 穿心莲、败酱草给药7天，随着给药剂量的加大，对小鼠外周血淋巴细胞DNA的损伤程更多还原

【Abstract】 In the present studies, the DNA damage of mouse lymphocyte cell induced by febrifuge and detoxifying herbs and their compatibilities frequently used clinically were studied with single cell gel electrophoresis （SCGE）, offering the evidence on heredity toxicology of the traditional Chinese medicine and guiding clinic rationally.In the study of comparing the whole blood specimen and abstracted lymphocyte in SCGE, the results showed that the lymphocyte DNA damage induced by those two noxious substances K₂Cr₂O₇ （10 mg/kg, 20 mg/kg, i.p.）and ethanol （1 g/kg, 2 g/kg, i.p.） could be found with the two methods indicating that whole blood specimen is more convenient than abstracting lymphocytes in SCGE.In the study of the DNA damage induced by the febrifuge and detoxifying herbs used alone, 1/3 × clinical dose, clinical dose and 3 × clinical dose were used in the experiments to detect the DNA damage of the lymphocyte once daily for 1d, 3d, 7d, respectively.（1） Flos Lonicerae and Portulaca Oleracea had no influence on periphery blood lymphocyte cell DNA in mice.（2） Brainea Insignis and Radix Isatidis could damage periphery blood lymphocyte cell DNA. The DNA damage induced by Brainea Insignis increased in a time-dose dependent manner.（3） When Caulis Lonicerae, Folium Isatidis, Flos Chrysanthem, Radix Pulsatillae Rhizoma Belamcandae and Herba Taraxaci were used once daily for 1 day; Brainea insignis, Paris Axixlis, Scutellaria更多还原