【作者】 丛悦；

【导师】 王金辉；

【作者基本信息】 沈阳药科大学 ， 药物化学， 2004， 硕士

【摘要】 益母草（Leonurus heterophyllus Sweet）为唇形科益母草属植物一年或二年生草本，广泛分布于欧亚大陆温带。其味辛、微苦，具有活血调瘀、利尿消肿之功效。在临床上用于月经不调，产后瘀阻、心脑血管疾病、血液病等疾病的治疗。 我们从益母草（Leonurus heterophyllus Sweet）地上部分的水提取物和醇提取物中，经溶剂分步萃取、大孔树脂、反复硅胶柱色谱、PHPLC和PTLC等方法得到11个化合物，并通过理化常数测定和光谱数据分析等手段鉴定为：山柰酚-3-O-β-D-[6-（4-羟基桂皮酰氧基）]吡喃葡萄糖苷（tiliroside，1），芦丁（2），槲皮素-3-O-β-D-（6-O-α-L-吡喃鼠李糖基）吡喃半乳糖苷（quercetin 3-O-robinobioside，3），megstigmane（4），益母草碱（5），ajugoside（6），2，6-二甲基-2E，7-辛二烯-1，6-二醇（7），丁香酸（8），苯甲酸（9），β-谷甾醇（10），胡萝卜苷（11），其中化合物3，4，7，8，9为首次从该属植物中分得。 由于益母草中含有很多具有一定活性的半日花烷型二萜，结合文献总结了半日花烷型二萜的¹³C NMR和¹H NMR波谱特征和规律。 采用大鼠离体子宫实验法，对益母草6个不同化学部位的增强离体子宫平滑肌收缩活动的活性进行了测定，发现总生物碱、极性酚酸类成分为其有效部位。更多还原

【Abstract】 Leonurus heterophyllus Sweet is a grass widely distributed in the Eurasia, which belong to Labiatae family. It possesses the functions of promoting blood circulation to remove blood stasis, detumescense and diuresis. It is available in curing irregular mensturation, puerperal congestion, cardiocerebrovascular diseases, blood diseases and so on.From the water extract, 95% ethanol extract and 70% ethanol extract of the aerial part of Leonurus heterophyllus Sweet, by solvent extraction, chromatograghic and recrystalllized methods, 11 compounds were isolated. On the basis of their physico-chemical properties and spectroscopic analysis, their structures were identified as tiliroside (1), rutine(2), quercetin3-O-robinobioside (3), megstigmane (4), leonurine (5), ajugoside (6), 2, 6-dimethyl -2E, 7-octadiene-1, 6-diol (7), syringic acid (8), benzoic acid (9), β-sitosterol (10), daucosterol (11). Among them, compounds 3, 4, 7, 8, 9 were isolated from this genus for the first time.Because of many labdane diterpenoids with some biological activities in this genus, a review on the NMR character of labdane diterpenoids was summarized based on the spectral data of the related references.The uterotonic properties of some different extracts are tested on isolated rat uterus, and the results showed that the crude alkaloids and phenolic acids were significant activities.更多还原