大青叶和板蓝根质量控制方法研究

【作者】 刘晓玲；

【导师】 孙国祥；

【作者基本信息】 沈阳药科大学 ， 药物分析， 2004， 硕士

【摘要】 本文研究了大青叶和板蓝根的高效液相色谱指纹图谱控制方法、定量分析方法以及大青叶的毛细管电泳指纹图谱控制方法，为二者的质量控制提供了新方法。 在建立大青叶和板蓝根的高效液相色谱指纹图谱时，分别测定了不同产地十批大青叶和板蓝根药材的高效液相色谱指纹图谱，以尿苷为参照峰，确立大青叶共有指纹峰为29个，板蓝根共有指纹峰为21个，以夹角余弦为测度分别对板蓝根和大青叶高效液相色谱指纹图谱进行了相关性评价，并以指纹图谱指数（F）和相对指数（Fr）对指纹图谱进行评价。对大青叶中的有效成分胞苷、尿苷、鸟苷和4（3H）-喹唑酮进行了定量分析，对板蓝根中腺苷、尿苷和4（3H）-喹唑酮进行了定量研究。 本文还对大青叶的毛细管电泳指纹图谱进行了方法学的研究，以胞苷为参照峰确立了18个共有峰，同样对其进行了指纹图谱评价。 本文就相同试验条件下的大青叶和板蓝根高效液相色谱指纹图谱进行了相关性研究，同时对大青叶和板蓝根红外光谱进行了研究，二者的红外指纹图谱具有明显的特征性和重现性。 本文研究了板蓝根和大青叶对内毒素的拮抗作用，以鲎试剂法测定二者的最低抗内毒素浓度均为31.25mg·mL^-1（以生药质量折算的表观浓度），不同批次的药材之间有显著差异，但是大青叶和板蓝根两种药材之间抗内毒素作用无明显差异。更多还原

【Abstract】 Reversed-phase high performance liquid chromatographic methods for the fingerprints of Folium Isatidis and Radix Isatidis and capillary electrophoresis (CE) method for the fingerprints of Folium Isatidis were investigated, which offered new methods on assessment of quality for both Folium Isatidis and Radix Isatidis.While we studied on the HPLC fingerprints, 29 co-possessing peaks were selected as the fingerprint peaks of Folium Isatidis and 21 co-possessing peaks were selected as the fingerprint peaks of Radix Isatidis. In both methods we chose uridine peak as the reference peak, and the fingerprints were evaluated by the chromatographic fingerprint index (F) and the relative index (Fr). Meanwhile, the methods of the quantitative determinations of cytidin, uridine, guanosine 4(3H)-quinazolinone in Folium Isatidis and adenosine, uridine, 4(3H)-quinazolinone in Radix Isatidis were developed.18 co-possessing peaks were selected as the fingerprint peaks of Folium Isatidis in the CEFP and the similarities between the chromatographic fingerprints of the herbal drugs were calculated by taking cytidin peak as the reference peak, and the fingerprints were also evaluated by the chromatographic fingerprint index (F) and the relative index (Fr).The correlation between the HPLC fingerprints of Folium Isatidis and Radix Isatidis was studied in the same conditions; meanwhile, IR fingerprints of the two were also studied, which showed obviously characteristic and reproducibility.The anti-endotoxin activity of Folium Isatidis and Radix Isatidis was investigated in this dissertation. There were significant differences of anti-endotoxin activity among different batches of herbal drugs, but no obvious differences between Folium Isatidis and Radix Isatidis.更多还原