【作者】 纪雪飞；

【导师】 邹莉波；

【作者基本信息】 沈阳药科大学 ， 药理学， 2006， 硕士

【摘要】 本论文研究了SY对小鼠移植性肿瘤生长的影响及其对荷瘤小鼠以及免疫功能低下小鼠免疫系统功能的影响，分别就SY对小鼠免疫器官、特异性免疫、非特异性免疫及细胞因子的作用进行了探讨。利用小鼠移植性肿瘤模型，考察了SY对肉瘤8₁₈₀、肝癌H₂₂及Lewis肺癌生长的影响。结果表明，SY60mg／kg～240mg／kg能够显著抑制3种瘤株的生长，抑瘤率均大于30％。SY能够显著提高小鼠抗疲劳及耐常压缺氧的能力并显著增加免疫器官指数。联合应用SY及化疗药环磷酰胺（CTX），观察其对小鼠肝癌H₂₂生长的影响，结果表明，联合用药组小鼠的瘤重显著低于单用环磷酰胺组，提示SY对环磷酰胺具有增效作用。SY对荷瘤小鼠免疫功能的影响实验结果表明，SY60mg／kg～240mg／kg显著增强小鼠脾T淋巴细胞增殖能力、NK细胞活性及IL-2活性并显著增强腹腔巨噬细胞体外吞噬中性红能力，改善荷瘤引起的小鼠的免疫抑制。利用治疗量的环磷酰胺造成免疫抑制模型，观察了SY对免疫功能低下小鼠迟发型超敏反应试验、血清溶血素生成试验、碳粒廓清试验各项指标的影响及其对化疗所致白细胞数量减少的影响。结果表明，SY能够显著改善环磷酰胺造成的小鼠免疫低下状态，显著对抗环磷酰胺所致的白细胞减少症。本研究还通过建立Lewis肺癌自发性肺转移模型，观察了SY对癌细胞转移的影响，结果表明，SY能够显著抑制Lewis肺癌的自发性肺转移。 上述结果提示，SY具有抑制肿瘤生长，对化疗的增效减毒作用以及显著提高机体免疫功能的作用，同时具有一定的抑制肿瘤转移的作用。更多还原

【Abstract】 In this paper we studied the effect of SY on growth of transplantable tumors. The effects of SY on immune system were also examined in mice bearing tumor or treated by cyclophosphamid （CTX）. We studied the effect of SY on the growth of sarcoma S₁₈₀ (S₁₈₀), hepatocellular carcinoma H₂₂(H₂₂) and Lewis Lung cancer （LLC）. The results showed that SY （60mg/kg²40mg/kg） significantly inhibit the growth of the three kinds of tumors and the inhibition rates are more than thirty percent. SY significantly promoted the anti-fatigue and anti-hypoxia ability and increased the spleen and thymus index in mice. We examined the effect of SY on the growth of H₂₂ in mice treated by lower dose CTX. The results showed that the tumor weights in the groups treated by CTX and SY were significantly decreased compared with the CTX group. The effects of SY on the immune system in mice bearing tumors were also studied and the results showed that SY significantly promoted the transformation of T lymphocyte stimulated by concanavalin A and increased the activity of interleukin （IL-2） and natural killer （NK） cell. The function of peritoneal macrophage was effectively increased compared with the control group. The therapeutic dose CTX was used to produce the depression of immune function, and we studied the effect of SY on immune system in model mice. The results showed that CTX could seriously produce the depression on the carbon clearance rate, the formation of serum hemolysin and the delayed type hypersensitivity and decrease the number of white blood cell and SY effectively turnover the depression caused by CTX. SY significantly inhibit the spontaneous pulmonary metastasis of LLC. All the results indicated that SY could significantly inhibit the growth of sarcoma S₁₈₀, H₂₂ and LLC and it suggested the potency as an anti-tumor medicine. SY could effectively promote the function of the immune system in mice bearing tumor and turnover the depression on the immune system induced by CTX in mice. The results indicated that the anti-tumor activity of SY could be related to the effect on the immune system and the further mechanism needs to be investigated.更多还原