【作者】 易钢；

【导师】 涂植光；

【作者基本信息】 重庆医科大学 ， 临床检验诊断学， 2006， 硕士

【摘要】 目的:通过绘制COHb和Hb的吸收光谱和一阶导数光谱,研究一氧化碳血红蛋白的导数光谱特性,并建立临床血液样品中一氧化碳血红蛋白的导数定量测定方法。方法:通过在血红蛋白溶液通入CO气可制备饱和COHb溶液,通入O2气后再加入Na2S2O4可制备饱和Hb溶液,以两饱和溶液作为测定COHb的相对标准溶液。采用日本岛津UV-265紫外-可见分光光度计获取样品的吸收光谱和导数光谱。结果:吸收光谱曲线表明,COHb的最大吸收波长在420nm左右,Hb的最大吸收波长在430 nm左右,两组分的光谱重叠十分严重,采用普通光谱分析方法难以对血液中COHb进行定量测定。通过绘制COHb和Hb的导数光谱,并利用导数光谱零交技术消除Hb干扰,发现在波长427nm的导数光谱值可应用于定量测定血液中一氧化碳血红蛋白。该方法可有效地消除Hb和基底物质的干扰,在COHb含量为0%～100%范围内具有优良的线性关系,对COHb>5%的样品,与双波长分光光度法测定结果具有良好的可比性。初步的临床应用表明导数光谱分析结果具有较好的精密度(cv≤3.11%)更多还原

【Abstract】 Objective:To study the derivative spectrogram characters of carboxyhemoglobin and establish a method for the quantitative determination of carboxyhemoglobin in blood with derivative spectrophotometry.Methods:COHb and Hb standard solutions were prepared with the pretreatment of carbonic oxide or oxygen ventilation followed by adding Na2S2O4. The derivative spectrophotometry was operated with Shimadzu UV-265 visible-ultraviolet spectrophotometer.Results:Absorption spectrum showed the maximum absorption wavelengthes (λmax) were 420nm for COHb and 430nm for Hb respectively, and both absorption spectrum of COHb and Hb were seriously overlaped. So that it was impossible to quantitative determine COHb in blood with routine spectrophotometry. However, it was found that the value of 1st derivative spectrophotometry at 427nm could be applied to test the COHb in blood quantitatively with zero-crossing technique. This method could effectively更多还原