【作者】 陈刚；

【导师】 孙世良； 李少林；

【作者基本信息】 重庆医科大学 ， 肿瘤学， 2006， 硕士

【摘要】 目的肿瘤的远处转移是导致恶性肿瘤死亡率高的重要原因,VEGF-C、VEGFR-3在此过程中起着非常重要的作用。本实验探讨了血管内皮生长因子C(VEGF-C)及其受体VEGFR-3(Flt-4,酪氨酸激酶受体-4)在乳腺癌组织中的表达对乳腺癌临床病理的意义。方法采用免疫组织化学染色方法,检测66例乳腺癌术后肿瘤组织标本中VEGF-C及VEGFR-3的表达,并结合临床病理参数,综合分析乳腺癌组织中VEGF-C的表达与患者年龄、肿瘤类型、肿瘤大小、淋巴结转移情况、临床分期、VEGFR-3的表达的相关关系。结果(1)VEGF-C定位于肿瘤细胞浆,呈棕褐色颗粒。VEGF-C只在乳腺癌组织中表达,而在邻近的正常腺组织和间质细胞中不表达。(2)VEGF-C在乳腺癌中表达阳性率为60.6%(40/66),flt-4的表达阳性率为53.0%(35/66);VEGF-C在有淋巴结转移组的表达阳性率为81.6%(31/38),在非淋巴转移组的表达阳性率为32.1%(9/28),在单一淋巴转移组的表达阳性率为80%(12/15)。(3)VEGF-C和VEGFR-3的表达具有相关性,相关关系较密切(p<0.005)(。4)VEGF-C的表达在淋巴转移组和非转移组之间具有显著性差异(p<0.005)(。5)在VEGF-C在非转移组、单一淋巴结转移组、多个淋巴结转移组的表达的两两比较中,非转移组和单一淋巴转移组之间具有显著性差异(p更多还原

【Abstract】 Objective: The high dead rate of malignant tumor mainly results from its local infiltration and metastasis. VEGF-C is a growth factor which is closely related to tumorigenesis and lymphatic metastasis in tumor. This study was to explore the correlation between the expression of VEGF-C and clinical pathological factors in breast cancer through the detection of the expression of VEGF-C and its receptor VEGFR-3.Methods: The expressions of VEGF-C and VEGFR-3 in 66 samples of breast cancer were detected by SP immunohistochemistry. And then the correlation between the expression of VEGF-C and clinical pathological factors was evaluated.Results: (1) The expression of VEGF-C is visible in the cytoplasm of cancer cells which is stained brown .The expression of VEGF-C is only located in breast cancer cells and cannot be detected in adjacent normal tissue and interstitial cells. (2) The positive rates of VEGF-C was 60.6%(40/66). The positive rates of Flt-4 was 53%(35/66). The positive rates of VEGF-C in lymph node metastasis team, non-lymph node更多还原