【作者】 王维；

【导师】 马传喜；

【作者基本信息】 安徽农业大学 ， 作物遗传育种， 2006， 硕士

【摘要】 选用三个有代表性的普通小麦品种作为亲本材料组配成2个单交组合(烟农19×皖麦48和烟农19×安农0016),用SDS-PAGE分析了F2代的高分子量麦谷蛋白亚基(HMW-GS)组成,测定了F2群体和F3群体的SDS沉降值,研究了F2代的高分子量麦谷蛋白亚基的分离,F2群体和F3群体的SDS沉降值的分布以及F2代的高分子量麦谷蛋白亚基对F2群体和F3群体的SDS沉降值的影响。主要结果如下:1各组合父本与母本的HMW-GS在F2中的出现情况是按一定的规律进行分离,它们的分离比因组合而异。组合Ⅰ烟农19×皖麦48:Glu-A1位点上的Glu-Ala(1)与Glu-Alc(N)的分离比为1:1;Glu-B1位点上的Glu-B1h(14+15)与Glu-B1i(17+18)的分离比为9:7。组合Ⅱ烟农19×安农0016:Glu-A1位点上的Glu-A la (1)与Glu-Alc(N)的分离比为3:1;Glu-B1位点上的分离比为1:1。Glu-B1i(17+18)和Glu-B1c(7+9)分别出现了仅显17号带和7号带的现象,因此编码17和18的两个基因以及7和9的两个基因并不是完全连锁,它们具有较低的重组率。2 F2代单株和F3代株系的SDS沉降值呈极显著正相关,因此SDS沉降值是育种早代选择的有效指标。3 F2群体HMW-GS的组成对F2群体和F3群体SDS沉降值有着显著的影响,且分析结果相似。F2群体Glu-A1位点内的变异和Glu-B1位点内的变异对F2单株、F3株系SDS沉降值的影响达到显著水平,Glu-A1与Glu-B1的互作效应不显著。两个杂交组合亚基的等位变异对SDS沉降值的影响大小顺序为:Glu-A11>Null,Glu-B17+9= Glu-B117+18> Glu-B114+15。4在不同的亚基组合类型中,具有1,7+9或1,17+18亚基的群体SDS沉降值高于具有N,14+15亚基的群体SDS沉降值。更多还原

【Abstract】 Two crosses (Yannong19×Wanmai48 and Yannong19×Annong0016) were made between three wheat (T. aestivum L.) varieties. The composition of High Molecular Weight Glutenin Subunits (HMW-GS) in F2 lines was screened by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The SDS sedimentation value of F2 and F3 lines was measured. The separation of HMW-GS in F2 lines, the distributing of SDS sedimentation value of F2 and F3 lines and the effect of HMW-GS on SDS sedimentation value were determined. The results were presented below:1 In the F2 lines, HMW-GS are separated in accordance with rules, which have related to the cross. In the crossⅠ(Yannong19×Wanmai48): The ratio of Glu-A1a to Glu-A1c was about 1:1; the ratio of Glu-B1h to Glu-B1i was about 9:7. In the crossⅡ(Yannong19×Annong0016): The ratio of Glu-A1a to Glu-A1c was about 3:1; the ratio in Glu-B1 was about 1:1. The incomplete expression has arisen in Glu-B1i and Glu-B1c, which will be very useful for wheat quality breeding. Therefore, two genes coding subunits 17 and 18, respectively, or two genes coding subunits 7 and 9, are incomplete linkage.2 The SDS sedimentation value of F2 lines significantly related to the value of F3 lines, so the SDS sedimentation value could be as a reliable quality criterion in the early generation selection of wheat quality breeding programs.3 The HMW-glutenin subunits of F2 lines have significant influences on SDS sedimentation value of the F2 and F3 lines and their influencing trends were almost same. The interaction between allelic of Glu-A1 and allelic of Glu-B1 did not reach significance. A sequence of effects of the HMW-glutenin subunits on the SDS sedimentation value of wheat was: Glu-A11>Null, Glu-B17+9=Glu-B117+18> Glu-B114+15.4 Genotypes with 1, 7+9 or 1, 17+18 performed higher SDS sedimentation value than that of genotypes with N, 14+15.更多还原