【作者】 刘志敏；

【导师】 徐立春；

【作者基本信息】 扬州大学 ， 中西医结合临床， 2006， 硕士

【摘要】 目的:通过检测莪术醇处理的胃癌SGC-7901细胞增殖的抑制率,细胞的形态学变化,DNA变化,以及凋亡抑制蛋白Bcl-2的表达,探讨莪术醇对胃癌SGC-7901细胞凋亡的诱导作用,初步阐明莪术醇的抗肿瘤机制,为莪术醇抗肿瘤的临床应用提供实验依据。研究对象:胃癌SGC-7901细胞株。方法:将莪术醇溶于无水乙醇,初始浓度为5mg/mL。设实验组A、B、C、D组,按比例浓度配制的12.5、25、50、100μg/mL莪术醇培养基,并以含1%无水乙醇培养基为阴性对照组E。采用MTT法和alamar blue法检测12.5-100μg/mL四种不同浓度莪术醇作用SGC-7901细胞24、48、72 h后细胞增殖的抑制率;采用AO/EB荧光染色法观察莪术醇处理SGC-7901细胞24、48、72h后细胞凋亡的形态学变化;采用PI标记流式细胞术检测莪术醇处理SGC-7901细胞24、48 h的凋亡率;采用Western Blot检测莪术醇处理SGC-7901细胞24、48 h后凋亡抑制蛋白Bcl-2的表达。结果:1、MTT法检测提示:12.5-100μg/mL莪术醇可以抑制细胞增殖,莪术醇对细胞增殖的抑制作用随莪术醇浓度增加和作用时间延长(除了12.5μg/mL莪术醇处理SGC-7901细胞24、48、72 h,三个时间段的抑制率之间无显著性差异(P>0.05))而逐渐增强,加药组的抑制率与对照组比较,有显著性差异(P<0.05)。2、alamar blue法检测提示:12.5-100μg/mL莪术醇可以抑制细胞增殖,莪术醇对细更多还原

【Abstract】 Objectives:To investigate the apoptosis effect of gastric cancer SGC-7901 treated by curcumol, inhibition ratio of proliferation of gastric cancer SGC-7901 cells, morphology changing, DNA changing and expression of important apoptosis protein Bcl-2 were detected. The results will be helpful for elucidating mechanism of curcumol anticancer, offering experimental data for clinic applying.Object:Human gastric cancer cells line: SGC-7901.Methods:Curcumol was dissolved in dehydrated alcohol and initial concentration of curcumol was 5mg/mL. Group A,B,C and D as experimental groups were set up .The curcumol concentrations were set as follows: 12.5、25、50、100μg/mL. Group E was control group, containing 1% dehydrated alcohol. After seeded for 24, 48 and 72h, inhibition ratios of SGC-7901 cells proliferation were detected by MTT assay and alamar blue assay, and morphology changings of apoptosis were detected by AO/EB fluorescent staining. After seeded for 24 and 48h, apoptosis ratios were detected by FCM and the expressions of bcl-2 were detected by Western Blot.ResuLts:1. 12.5-100μg/mL curcumol could restrain proliferation of SGC-7901 after treated for 24, 48 and 72h detected through MTT assay. With the increase of curcumol concentration and the incubation time, the inhibition was reinforced. Comparing to control group, there更多还原