【作者】 吕爱玲；

【导师】 张福；

【作者基本信息】 天津科技大学 ， 化学工艺， 2005， 硕士

【摘要】 极端嗜盐绿色杜氏藻的藻体内有较为特殊的活性物质,具有极强的抗逆性。它对盐度、温度、pH值、光照和药物等有着极强的适应能力,具有潜在的开发应用前景。 本课题主要对中国渤海滩涂极端嗜盐绿色杜氏藻的生物学特征进行了初步探索。内容包括绿色杜氏藻的耐盐特性、繁殖特性、生长特性、以及对卤水生境因子（包括化学因子,物理因子,生物因子）适应性的研究,并初步调查了自然条件下在渤海滩涂上绿色杜氏藻的生存发展的规律。 实验结果表明: 绿色杜氏藻需要一定的盐度才能正常生长,对盐度的适应范围广。对高渗震动的适应比对低渗变化的适应速度慢。实验室养殖适宜的盐度为3°Be′～8°Be′。最大生长量和生长率都出现在3°Be′和5°Be′组,最高藻密度达6000×10⁴cells/ml。自然界中常在高浓卤水中（>15°Be′）成为浮游植物中的优势种类。饱和卤水有利于保种。 绿色杜氏藻有三种生长方式:光合自养、异养和混合生长,其中以混合生长最好。绿色杜氏藻可进行营养繁殖、无性繁殖和有性繁殖,具有特殊的细胞破碎繁殖方式。 绿色杜氏藻在自然和普通光源下皆能生长,在黑暗环境中也能生存。24小时的持续强光照使藻的生物活性降低,适当给予一定的黑暗期有助于其生长。培养液中加入卤虫干粉发酵液,养殖中期补充2～3g/L NaCl、1～2g/LMgCl₂,后期添加5～6g/L Na₂SO₄,将促进绿色杜氏藻的生长。更多还原

【Abstract】 There are comparatively special active materials in the body of the extreme halotolerant Dunaliella viridis. D.viridis has extremely strong ability to resist against extreme environment. It has strong adaptable capacities to salinity, temperature, pH, light and druggery. It has potential development and application prospects.Several preliminary experiments were conducted to find the biological characteristics of the extreme halotolerant Dunaliella viridis living in the mud flat of Bohai Sea Gulf of China. Its contents included the study on tolerant characteristic to salinity, reproduction characteristic, growth characteristic, adaptable ability to environmental factor of high enriched brine’s （including chemical factor, physics factor, biological factor） and the survival and development law of D.viridis in the mud flat of Bohai Sea Gulf was investigated.The results of the experiments show as follows:D.viridis can grow normally in certain salinity and adapts wide salinity range. The speed of adaptation to hypoosmotic shock is lower than to hyperosmotic shock. Suitable salinity of laboratory is 3°Be’ ～8°Be’ The maximum growth content and rate of D.viridis are obtained at the salinity 3°Be’ and 5°Be’ . The maximum growth content reaches 6000× 10⁴cells/ml. D.viridis usually becomes predominant species in high enriched brine （>15°Be’ ） in the nature. The saturation brine helps to save the algae seed.D.viridis has 3 kinds of Growth mode: photosynthesis autotrophy, growth in the dark condition and mixed growth. Mixed growth is the best. D.viridis has nutrition, vegetative, sexual propagation, and a special fragmentized cell reproduction mode.D.viridis can grow under natural and common lights and survive in the dark environment. Its biological activation will depress under 24 hours strong illumination condition and growth will be accelerated if given properly certain darkness period. To promote its growth, fermented liquid of Artemia dry powder should be added to culture medium and the nutrition salt 2³g/L NaCk 1～2g/L MgCl₂ should be added to culture liquid in the middle periods and 5～6g/LNa₂SO₄ should be added in the later periods.更多还原