【作者】 王冬英；

【导师】 黄维义； 张为宇；

【作者基本信息】 广西大学 ， 预防兽医学， 2006， 硕士

【摘要】 本文采用胆囊检查法对广西水牛和黄牛片形吸虫的感染情况进行了调查，并对片形吸虫在中间宿主体内的发育过程以及三种片形吸虫的形态学及PCR鉴定进行了研究。 从屠宰场收集水牛和黄牛胆囊，在收集胆囊的过程中粗略地观察肝脏病变，胆囊检查主要检查胆囊中是否有片形吸虫虫体或虫卵，以及胆囊是否有病变，结果发现有虫或虫卯的判为阳性，有眼观病变但无虫及虫卵的定为可疑，无眼观病变且无虫卯的为正常。结果水牛阳性率为57.24％，可疑28.96％，正常13.8％；黄牛阳性率为52.8％，可疑为7.87％，正常为39.33％。 片形吸虫在中间宿主体内的发育情况研究：三种片形吸虫毛蚴分别感染广西小土蜗螺，每只螺感染1～5条毛蚴，另设对照组，感染前后用去氯离子水饲养，用生菜饲喂。结果在水温20～28℃(室温22.5～32℃)，其中有两天为16，17℃时，对照组和感染肝片形吸虫组的没检查到各期幼虫，感染大片形吸虫和中间型片形吸虫毛蚴的在感染后28天见螺体内有自由的尾蚴，34～36天开始有尾蚴从螺体逸出。 在种类鉴别方面，可以根据虫体的形态将片形吸虫大体分为三类：肝片形吸虫，大片形吸虫和中间型片形吸虫。ITS-2特异PCR也已初步证实更多还原

【Abstract】 The objectives of the present study were carried out to determine the epidemiology of fascioliasis in water buffaloes and cattle by examined the gallbladder, study the life cycle of Fasciola spp in Galba pervia and identify the Fasciola spp by morphological and PCR.Gallbladders were obtained from the local abattoir, the pathological changes of the livers were observed during collected the gallbladders. The gallbladder examination was chiefly to check if there were liver worms or eggs in the gallbladder. The results was determined as follows: if flukes or eggs were founded in the cholecyst, it were signed Positive ; those which had pathological changes but no flukes or eggs were signed suspicious, and those no pathological changes , flukes or eggs were considered as negative.The results shew that 57.24% was positive, 28.96% was suspicious, only 13.8% was negative in water buffaloes. In the cattle the positive,suspicious and negative rate was 52.8%, 7.87% and 39.33% , respectively.The life cycle of Fasciola species in snails: three groups of Galba pervia were infected with 1～5 Fasciola hepatica, Fasciola gigantica and the更多还原