【作者】 邓椋爻；

【导师】 刘丕庆；

【作者基本信息】 广西大学 ， 作物遗传育种， 2006， 硕士

【摘要】 水稻白叶枯病（Rice Bacterial Blight，BB）和稻瘟病（Rice Blast）广泛分布于水稻栽培区，是水稻上最重要的病害之一。利用具有广谱抗性的抗源和抗性基因进行抗病育种是当前控制水稻白叶枯病和稻瘟病的关键措施。编号386的品种是含有广谱抗稻白叶枯病Xa23基因的材料。编号105的品种是含有南方主要抗稻瘟病Pi1、Pi2基因的材料。 应用与Xa23基因紧密连锁的SSR标记RM206对已转入Xa23基因的三个组合（384×386）、（385×386）和（387×386）的BC₂、BC₃群体以及其F₄群体进行分子检测。成功筛选出具有RM206标记片段的植株，为改良稻白叶枯病抗性育种提供了原始材料。 105号品种分别与具有优良性状的待改良材料582和84号杂交F₁，自交F₂、F₃；并利用582作轮回亲本获得BG群体。选用与Pi1连锁的SSR标记RM144，与Pi2紧密连锁的SSR标记SRM24对分离群体进行跟踪选择。分别在各分离群体中检测到同时含有两个标记的基因聚合植株，丰富了水稻抗稻瘟病材料。 采集广西区南部地区的下雷和玉林的稻瘟病病菌，对编号为47、105、106的三个水稻材料进行接种鉴定。结果表明，47号和105号品种对广西区南部的部分稻瘟病病菌具有一定的抗性，但抗性不稳定。更多还原

【Abstract】 Rice Bacterial Blight （BB） and Rice Blast （RB） are the most serious rice diseases and widely distributed in the rice cultivation areas. At present, using broad spectrum resistant sources and genes are the key means to improve the rice resistance to BB and RB. NO.386 variety includes the resistant gene Xa23 to BB. NO.105 variety includes main resistant genes Pi1 and Pi2 to RB.SSR marker RM206 is closely-linked to gene Xa23 and used to make molecular identification in （384x386）, （385x386） and （387x386） BC₂, BC₃ and F₄ populationsJt’s successful to select the plants which contain the segments of RM206, and provide resistant source materials to BB.The No.582 and No.84 have good agronomic characters.NO.105 variety was hybridized separately with No.582 and No.84 to produce F₁, inbred line F₂, F-3. And No.582 was used as the return parent to obtain the （582x105） BC₁ populations. Molecular track was carried out in the separated breeding populations with SSR markers RM144 and SRM24.The RM144 is linked to Pi1,the SRM24 is closely-linking to Pi2.The pyramiding-genes plants that contained the segments of RM144 and SRM24 concurrently was selected to enrich the resistant materials of rice blastRice blast local pathogens of Xia Lei and Yu Lin were gathered in southern of Guangxi. The NO.47, NO. 105 and NO.106 were inoculated by the local pathogens to identify their rice blast resistance.The results showed that NO.47 and NO.105 had a certain resistance to same parts of Guangxi local pathogens. But,their resistances were not steady.更多还原