【作者】 王杨；

【导师】 陈茂怀；

【作者基本信息】 汕头大学 ， 病理学与病理生理学， 2006， 硕士

【摘要】 背景和目的 鼻咽癌在东南亚一带是高发恶性肿瘤，尤其多见于我国的广东省，在广东省鼻咽癌约占全身恶性肿瘤的32％。与其他头颈部肿瘤相比鼻咽癌更倾向于在早期发生浸润、转移，常常是在原发灶发现肿物前就已经发生临床转移，颈部淋巴结转移大是90％鼻咽癌患者的首发症状。鼻咽癌的浸润、转移机制是复杂的，它涉及肿瘤细胞本身与宿主之间错综复杂的关系，研究人员通过研究蛋白分解酶、血小板、瘤内血管生成及相关因子等在鼻咽癌转移中的作用并筛选出一些鼻咽癌转移相关的癌基因、以期明确鼻咽癌高转移性的发生机制，但至今为止，国内外临床工作者仍缺乏有效预测鼻咽癌侵袭和转移倾向的指标。在基质金属蛋白酶家族(MMPs)与鼻咽癌转移的关系上，目前的研究仅限于报道MMP-2，MMP-9等几个指标，而基质金属蛋白酶家族诱导因子(EMMPRIN)与鼻咽癌转移的关系及EMMPRIN、基质金属蛋白酶抑制物(TIMPs)在鼻咽癌浸润转移过程中与MMPs的相互关系的研究在国内外均未见报道。为研究EMMPRIN，MMPs，TIMPs在鼻咽癌转移过程中的相互关系，我们应用免疫组织化学技术及原位杂交技术检测了无转移鼻咽癌及伴颈部淋巴结转移鼻咽癌组织中MMP-2、MMP-9、EMMPRIN、LMP-1及TIMP-2的表达情况，以期揭示它们在鼻咽癌转移中的作用及其相互关系以及鼻咽癌细胞转移的分子生物学机制，从中筛选具有预测鼻咽癌转移倾向的分子生物学指标，为临床提供反映鼻咽癌侵袭、转移的辅助性诊断指标。更多还原

【Abstract】 Background and objectiveNasopharyngeal carcinoma (NPC) has its highest incidence rate in Southern China and is one of the most common cancers in Southeast Asia. It is a highly metastatic and invasive malignant tumor. NPC often spreads out clinically before forming a mass in the primary lesion. Essential steps in the process of tumor invasion and metastasis include the degradation of the extracellular matrix (ECM) and basement membrane (BM). The invasion of the BM by tumor cells is thought to be one of the critical steps in metastasis. Many proteolytic enzymes degrade components of the ECM and BM. Among these, the matrix metalloproteinases (MMPs) are critical for tumor cell invasion and metastasis. Previous experimental and clinicopathological studies have revealed a good correlation between expression of MMP1, MMP2, MMP9 and NPC. The relationship between expression of EMMPRIN and cervical metastasis in NPC has not been studied. The role of MMP-2, MMP-9, LMP-1, TIMP-2 and their relationships between each other during NPC metastasis have not been published. To make the mechanism of NPC metastasis clear and screen some molecular biology makers which could predict NPC invasion, In our study we use immunohistochemitry and in situ hybridization to test the expression of EMMPRIN, MMP-2, MMP-9, LMP-1 and TIMP-2.Materials and Methods1 Tissue Samples. 53 specimens were obtained from patients with NPC who underwent biopsy at the Shantou University Tumor Hospital and Shantou central hospital from 1996 to 2003. All the specimans were divided into three groups: 15 cases chronic nasopharyngitis (CN group); 20 cases of no metastasis NPC (NM-NPC group, tumour stages were classified according to the TNM classification of the International Union against Cancer); 33 cases of lymph node metastasis NPC (LNM-NPC group), it worth to point out that they were paired cases both with tissues from primary tumor and its metastatic tumor in cervical lymph node. All cases were diagnosed by veteran pathologist or clinical doctor. Forty-one patients were male, and twelve patients were female (mean age, 51.9 years; range, 30-76years). Specimens were fixed in 10% formaldehyde solution and embedded in paraffin.更多还原