藏药红景天及其复方抗缺氧有效成分研究

【作者】 王毓杰；

【导师】 张艺；

【作者基本信息】 成都中医药大学 ， 药物化学， 2006， 硕士

【摘要】 藏药复方缺氧康是藏医大师措如·才朗的经验方，方中红景天为主药，辅以青藏龙胆、甘草等藏医治疗高原缺氧的常用药物。具有活血，清肺，益气安神的功效。用于治疗高原缺氧引起的恶心，呕吐，胸闷，倦怠气喘。该方已在临床使用多年，疗效确切。 本文通过整体动物试验、细胞生物学试验，筛选出红景天及其复方抗缺氧的有效部位，并采用植化研究方法和HPLC指纹图谱分析技术，初步阐明红景天及其复方缺氧康治疗高原缺氧有效组分。 1 红景天及其复方抗缺氧有效部位筛选研究 对主药红景天的不同溶剂部位和复方不同工艺制备的提取物，采用整体动物和心肌细胞活力筛选研究。结果表明：红景天的乙酸乙酯部位、正丁醇部位、水部位以及复方缺氧康的大孔树脂部位有较好的抗缺氧作用。 2 红景天抗缺氧有效成分研究 通过系统溶剂法、柱色谱法和制备薄层色谱法，从红景天的乙酸乙酯、正丁醇两个有效部位中分离得到10个化合物，鉴定了其中8个化合物的结构，分别为：红景天苷、酪醇、没食子酸、德钦红景天苷、大花红景天素、β—谷甾醇、胡萝卜苷、二十六烷醇。 对部分化合物进行心肌细胞活力实验，结果表明：红景天中红景天苷、酪醇、没食子酸、德钦红景天苷、都有不同程度的促进缺糖缺氧心肌细胞活力的作用。 3 红景天及其复方缺氧康抗缺氧有效组分指纹图谱初步研究 通过建立红景天的抗缺氧有效部位的指纹图谱，对其中的主要化学成分进行归属。建立抗缺氧作用最强的复方缺氧康大孔树脂部位以及复方中各单味药材的指纹图谱，通过色谱峰的保留时间和紫外光谱图，对该部位中的成分进行对应药味的归属。初步阐明复方缺氧康的有效组分为：红景天苷、酪醇等酚类化合物、龙胆苦苷等裂环环烯醚萜类化合物以及青藏龙胆、甘草和烈香杜鹃中的黄酮类化合物。 4 藏药复方缺氧康胶囊质量标准研究 在初步阐明缺氧康药效物质基础的前提下，对藏药复方缺氧康胶囊进行质量更多还原

【Abstract】 Que-Yang-Kang which is a Tibetan compound prescription is an experiential recipe ofi Tibetan medical master-Cuoru ? Cailang. Primary ingredients in the prescription is Radix etRhizoma Rhodiolae. And the adjuvant includes several common herbs treated anoxia in altiplano such as Flos Gentianae(Gentiana przewalskii Maxim.). Que-Yang-Kang possess of the efficiency of supplement Qi for activating Blood circulation, dredge the meridian and relieve asthma. It is used to cure Blood stagnation due to Qi Deficiency, precordial pain due to obstruction of Qi in the chest, hemiplagia due to apoplexy, fatigue and asthma.The article has screening the anti-anoxic effective fraction of Radix et Rhizoma Rhodiolae and Que-Yang-Kang compound prescription by the means of laboratory animal experiments and measurement to activity of cells. Then phytochemical research methods including extraction, separation and purification, and high performance liquid chromatograph fingerprinting technologies have been carried out, aimed at illuminating preliminary the effective subsistence basis against anoxia in altiplano in Radix et Rhizoma Rhodiolae and Que-Yang-Kang compound prescription.1 Anti-anoxic pharmacological screening researches of Radix et Rhizoma Rhodiolae and Que-Yang-Kang compound prescriptionIt has been carried out that the anti-anoxic pharmacological screening researches to different solvents extracting fraction of Radix et Rhizoma Rhodiolae and different extracts of Que-Yang-Kang compound prescription produced by different preparing routing. The result is disclosed that ethyl acetate extracted fraction, normal butyl alcohol extracted fraction, and water fraction of Radix et Rhizoma Rhodiolae and alcohol extracting fluid of Que-Yang-Kang separated and purified by macroporous resin possessed of anti-anoxic activity obviously.2 Studies on anti-anoxic effective components of Radix et Rhizoma RhodiolaeBy the means of systematic solvent extraction, column chromatograph and preparative thin layer chromatograph separation, 10 compounds have been gained by separated and purified from the two effective extracted fraction of ethyl acetate extracted fraction and normal butyl alcohol extracted fraction. And the structures of 8 compounds have been identified. They are salidroside,更多还原