初探黄斑康对实验性血脂异常小鼠巩膜顺应性的影响

【作者】 高海燕；

【导师】 曾庆华；

【作者基本信息】 成都中医药大学 ， 中医眼科学， 2006， 硕士

【摘要】 目的：初步探讨探黄斑康对实验性血脂异常小鼠巩膜顺应性的影响。 方法：选用C57BL小鼠，喂养普通饲料一周后，采用单纯随机抽样法抽取6只，采血检测血脂、血液流变学和血清丙二醛。剩余动物给予高脂饲料五个月后，单纯随机抽样法随机抽取6只，采血检测血脂、血液流变学和血清丙二醛，确认模型成功。将余下动物按体重随机分为5组：阴性对照组(12只)、阳性对照组(12只)、低剂量治疗组(12只)、中剂量治疗组(12只)、高剂量治疗组(12只)。各组给予不同的药物灌胃1个月，随后处死各组动物作各项指标的检测，同时摘取眼球作光镜和电镜的病理切片。 结果：(1)黄斑康治疗各组血脂、血液流变学、丙二醛指标均低于阴性对照组、阳性对照组(P＜0.01)；黄斑康治疗组内，中剂量治疗组明显低于低、高剂量治疗组，与低、高剂量治疗组比较有显著性差异(P＜0.05)；低剂量治疗组与高剂量治疗组比较无统计学意义(P＞0.05)；(2)五组间胶原纤维平均光密度值比较无统计学意义(P＞0.05)；(3)黄斑康治疗各组巩膜Ⅰ型、Ⅲ型胶原纤维IOD值均低于阴性对照组、阳性对照组(P＜0.01)；黄斑康治疗组内，中剂量治疗组明显低于低、高剂量治疗组，与低、高剂量治疗组比较有显著性差异(P＜0.05)；低剂量治疗组与高剂量治疗组比较无统计学意义(P＞0.05)；(4)黄斑康治疗各组巩膜ColⅢ／ColⅠ面积比均高于阴性对照组、阳性对照组(P＜0.01)；黄斑康治疗组内，中剂量治疗组明显高于低、高剂量治疗组，与低、高剂量治疗组比较有显著性差异(P＜0.05)；低剂量治疗组与高剂量治疗组比较无统计学意义(P＞0.05) 结论：中药黄斑康能有效降低血脂异常小鼠血脂、丙二醛水平，改善血液流变学状态；能够通过改善实验性血脂异常小鼠巩膜顺应性来防治年龄相关性黄斑变性的发生，而且疗效以中剂量为佳。更多还原

【Abstract】 Object: To observe the effect of Huangbankang on sclcral compliance. And expore the relationship between Age-Related Macular Degencration and the effect.Methods: Choosing C57BL mice, after a week of normal forage, six mice were selected randomly and killed for testing TC、 LDL-C and MDA.The rest mice fed with high-fat dietary for five months.Then select six mice randomly to kill for testing TC、 LDL-C and MDA. The rest mice were divided randomly into 5 groups on the basis of weight: the negative control group (twelve mice) ; the positive control group ( twelve mice ) ; The Huangbankang treated group of lower-dosage; The Huangbankang treated group of middle-d(?)age; The Huangbankang treated group of higher-dosage. They were executed and ch(?)ked the test index, take off all their eyeballs to make pathological section.Results: (1) The blood biochemistry index of the Huangbankang treated groups are lower than others (P<0.01) ; In the Huangbankang groups, the middle-dosage all lower than others (P<0.05), but between the lower-dosage and the higher-dosage, the index has no difference (P>0.05) ; (2) The mean light density of the five groups have no difference (P> 0.05) ; (3) The Collagen I ’s and CollagenIII’s IOD of Huangbankang treated groups are lower than others (P<0.01); In the Huangbankang groups, the middle dose all lower than others (P<0.05), but between the lower-dosage and the higher-dosage, the index has no difference (P> 0.05) ; (4) The ColIII/Col I of Huangbankang treated groups are higher than others (P<0.01); In the Huangbankang groups, the middle dose all higher than others (P <0.05), but between the lower-dosage and the higher -dosage, the index has no difference (P>0.05) ;Conlusion: The Huangbankang could significantly lower the blood fat and MDA in mice, improve hemorheology condition; and protect the ARMD belong lower the scleral compliance.更多还原