电针治疗急性痛风性关节炎的血管通透机制研究

【作者】 崔莉；

【导师】 刘旭光；

【作者基本信息】 成都中医药大学 ， 针灸推拿学， 2006， 硕士

【摘要】 目的：观察电针对急性痛风性关节炎模型家兔关节周径的影响，把血管通透功能作为切入点，比较5-HT、HA、LTB₄和NO四种物质各组间的含量差异，探讨电针治疗急性痛风性关节炎的部分作用机制。 方法：以家兔为实验对象，将尿酸钠溶液注射于兔双膝关节造成急性痛风性关节炎动物模型，根据“清热化湿、健脾益肾”的治疗原则，电针“足三里”、“三阴交”穴，每日一次，连续治疗7天。比较各组间造模前、造模后及治疗后的关节周经，并于治疗7天后，处死动物，取关节滑膜组织及滑膜液，采用荧光测定、酶联免疫、硝酸还原等方法，检测局部炎症介质组胺（HA）、5-羟色胺（5-HT）、白三烯（LTB₄）和血管舒张因子一氧化氮（NO）含量。 结果：（1）与模型组比较，电针治疗组动物关节周径显著减小（P<0.05）；（2）与模型组比较，电针治疗组动物关节局部组胺（HA）、5-羟色胺（5-HT）、白三烯（LTB₄）和血管舒张因子一氧化氮（NO）含量显著降低（P<0.01）。 结论：电针对急性痛风性关节炎模型有抗炎消肿作用，能减小关节周径，能明显降低关节滑膜组织炎症介质组胺（HA）、5-羟色胺（5-HT）、白三烯（LTB₄）和血管舒张因子一氧化氮（NO）含量。更多还原

【Abstract】 Objection: Observe effect of electric-accupuncture therapy with arthritis perimeter of rabbits model with Acute Gouty Arthritis. Compare the content of 5-HT, HA, LTB₄ and NO among three groups, explore the mechanism of electric-accupuncture therapy on acute gouty arthritis partly.Method: Made the acute gouty arthritis rabbits model by injecting sodium urate crystal into both knee joints of rabbits, with the principal of "removing Heat and Dampness, strengthening Spleen and tonifying Kidney" , acupunctured "Zusanli" and "Sanyinjiao" points with electric once daily lasting seven days,measured and compare the perimeter of articular genu before making model, after making model and after treatment among three groups, got membrana synovialis and synovial fluid after seven days killing the animals. Discoved the content change of Histamine, Serotonin , Leukotriene, vasoditation factor nitrogen monoxidum and the of synovial membrance with Fluorimetry, Enzyme Immunoassay and Nitric Acid Reduction.Result: Firsly, perimeter of articular genu of rabbits in treatment group is reduced more obviously than that in model group （P P<0. 05） , Secondly,the content of inflammation mediator, HA, 5-HT, LTB4 and vasoditation factor NO were reduced with electric-accupuncture therapy, the change of group of electric-accupuncture therapy had obvious significance compared with model group , P<0.01.Conclusion: Electric-accupuncture therapy can retrieve permeability of local arthritis of rabbits suffering acute gouty arthritis and engorgement statement. The contents of HA, 5-HT, LTB4, vasoditation factor NO were reduced with electric-accupuncture therapy.更多还原