滋肾丸抗去势雌鼠骨质疏松症的实验研究

【作者】 陈红；

【导师】 杨仁旭；

【作者基本信息】 成都中医药大学 ， 中医内科学， 2006， 硕士

【摘要】 目的 探讨补肾中药滋肾丸对去卵巢骨质疏松模型大鼠股骨的密度、骨矿含量、生物力学参数的影响及机制。 方法 将66只15周龄SD大鼠随机分为6组，每组11只，空白组做假手术，其余5组做卵巢切除术，术后2周开始给药，用药12周后处死，测定右侧股骨的骨密度及骨矿含量、腰一椎体骨生物力学参数、血清雌二醇、碱性磷酸酶含量，子宫湿重。 结果 模型大鼠股骨的体重标准化骨密度、骨矿含量明显下降，腰一椎体骨生物力学参数值下降，血清碱性磷酸酶升高，血清雌二醇下降，子宫指数减轻。滋肾丸大、中、小剂量组、尼尔雌醇组能防止骨密度、骨矿含量的下降(与模型组相比P＜0.05)。滋肾丸大、中剂量组能防止腰一椎体骨生物力学值的下降(与模型组相比P＜0.05)，小剂量组的参数较模型组亦有提高，其值与空白组已无显著差异，但与模型组的差异亦无显著意义。滋肾丸各剂量组、尼尔雌醇组均能防止血清碱性磷酸酶的升高，血清雌二醇的下降(与模型组相比P＜0.05)，滋肾丸中剂量组、尼尔雌醇组能防止子宫指数的减轻(与模型组相比P＜0.05)。 结论 补肾中药滋肾丸对去卵巢骨质疏松模型大鼠股骨的密度、骨矿含量，骨生物力学参数有正向影响，其机制与其升高血清雌二醇，降低血清碱性磷酸酶含量进而抑制过高的骨转换以及促进骨形成有关。更多还原

【Abstract】 Objectives To investigate the effect and the mechanism of kidney-reinforcing Chinese herb Zishenwan on osteoporosis of ovariectomized rats.Methods Female SD rat(n=66,15 weeks old) were randomly assigned to 6 groups of 11 each., the blank group were shamming operated; the other groups were performed surgery of removal both ovary .Medicines were given 2 weeks after surgery, which last 15 weeks and then killed. The right thighbone were taken out, and the BMD, BMC determined; The first waist centrum was taken out, and its biomechanics parameter determinded; the blood were collected, and E2 and ALP were determined; the ovary were taken out, and be weighted.Results The BMD/kg, BMC/kg of right thighbone and the biomechanics parameter decreased evidently, the level of ALP increased, the level of E2 and the weight of ovary decreased evidently in model group. Zishenwan group as well as western medicine control group can antagonize these changes which has the statistics meaning(P<0.05).Conclusions kidney-reinforcing Chinese herb ZiShenwan can improve the BMD/kg, BMC/kg, biomechanics parameter of ovariectomized osteoporosis rat, the mechanism has relationship with its raising the level of E2 in blood and reducing the level of ALP to depress the high bone turnover and improve the bone formation.更多还原