【作者】 侯云英；

【导师】 范秀珍；

【作者基本信息】 山东大学 ， 护理学， 2006， 硕士

【摘要】 目的：观察连续3天中等强度运动训练对大鼠缺血／再灌注(I／R)心肌的保护作用；以及运动的心脏保护效应与蛋白激酶C(PKC)和锰超氧化物歧化酶(MnSOD)的关系。 方法：通过筛选实验的Wistar大鼠随机分为4组(每组18只)。①运动组(Exe组)：铁皮游泳池内游泳适应3天，每天20min，之后连续游泳3天，每天1h，每次游泳前10min经腹腔注射生理盐水1ml；②对照组(Con组)：正常饲养，每天腹腔注射生理盐水1ml；③运动+PKC抑制剂组(E+C组)：每次游泳前10min经腹腔注射PKC抑制剂CHE 2 mg／Kg，余同Exe组；④PKC抑制剂组(CHE组)：每天腹腔注射CHE 2 mg／Kg，余同Con组。以上4组大鼠每组再随机分为2组：(A)I／R组(每组10只)：最后一次运动训练后24h，建立离体心脏Langendorff-I／R模型，全心缺血30min，再灌注60min，观察各组大鼠再灌注期间心功能指标(HR、LVDP、RPP)和冠状动脉流量(CF)恢复率，心律失常的发生情况及心肌梗死范围；(B)假处理组(每组8只)：最后一次运动训练后24h，取左心室心肌样品进行超氧化物歧化酶(SOD)活性测定。 结果： 1．Exe组大鼠LVDP(再灌注30、60min)和RPP(再灌注20、30、60min)的恢复率高于Con组和E+C组(p＜0.05)，心肌梗死范围显著低于Con组和E+C组(p＜0.01)；CHE组、Con组上述各指标差异无统计学意义(p＞0.05)。各组心律失常评分和HR、CF恢复率差异无统计学意义(p＞0.05)。 2．Exe组大鼠MnSOD活性高于Con组和E+C组(p＜0.05)；CBE组、Con组MnSOD活性差异无统计学意义(p＞0.05)。各组总SOD(T-SOD)和铜-锌SOD(Cu-ZnSOD)活性差异无统计学意义(p＞0.05)。更多还原

【Abstract】 Objective: To oberserve the protective effect of 3 consecutive days of moderate exercise on rat ischemic/reperfused myocardium and its correlation to protein kinase C (PKC) and Manganese Superoxide Dismutase(MnSOD)Methods: Wistar rats which passed the screening test were randomly divided into 4 groups: ①exercise group (Exe group): a group that received exercise pretreated with water for injection (1 ml) as a vehicle. The exercise protocol is that rats swam in an iron pool for 3 days, 1 hour per day, after 3 days of adaptation, 20 minutes per day. ②control group(Con group): a group that dit not exercise and was fed normally treated with water for injection (1ml). ③exercise + PKC inhibitor group(E+Cgroup): a group that received exercise pretreated with chelerythrine chloride 2 mg/Kg intraperitoneally for injection, 10 minutes before each exercise session. ④ PKC inhibitor group(CHE group): a group that was fed normally and treated with chelerythrine chloride. Each group of the above 4 groups were divided into 2 groups randomly: (A) I/R group (10 rats each group): Langendorff-ischemia/reperfusion model was made in isolated rat heart in vitro 24 hours after the last swimming session, to observe the recovery of cardiac function(HR、 LVDP、 RPP), occurrence of arrhythmia, and infrct size. (B) Sham treated group (8 rats each group): ischemia/reperfusion model was not made, but the activity更多还原