【作者】 王秀梅；

【导师】 黎莉；

【作者基本信息】 山东大学 ， 肿瘤学， 2006， 硕士

【摘要】 研究背景：角质细胞生长因子(keratinocyte growth factor，KGF)是上皮细胞特异的促有丝分裂剂，是在1989年由Rubin等人从人胚胎性肺成纤维细胞的生长培养液中分离出来的。KGF的cDNA序列表明它属于成纤维细胞生长因子(FGF)家族，即FGF-7。KGF来源于间质细胞，与上皮细胞表面KGF受体(KGFR)特异性地结合，以旁分泌的方式刺激上皮细胞有丝分裂。间质细胞和上皮细胞的这种相互作用参与多种组织、器官如皮肤、胃肠、呼吸道、生殖系统的形成和发育。间质和肿瘤细胞的相互作用是肿瘤细胞发生侵袭、浸润和转移的重要因素。近年来KGF与肿瘤的关系成为研究的热点。 研究目的：探讨KGF蛋白在人乳腺癌和正常乳腺组织中的表达；KGF的表达与临床病理因素及某些生物学指标的相关性；研究KGF在乳腺癌表达的临床意义。 研究方法：采用免疫组化的方法检测63例乳腺癌组织和30例正常乳腺组织中KGF的表达，同时检测乳腺癌组织中ER、PR、CerbB-2和Ki-67的表达，分析KGF在肿瘤细胞的表达与患者的年龄、肿瘤大小、浸润程度、淋巴结转移、TNM分期、分化程度及某些预后指标的相关性，采用SPSS12.0 for windows统计软件包进行数据处理。 研究结果：63例乳腺癌组织中34例肿瘤细胞胞质表达KGF(54.0％)，正常乳腺上皮细胞不表达，两者间差异有显著统计学意义(P＜0.01)。乳腺癌间质细胞和正常乳腺组织间质细胞KGF的表达率分别为34.9％和16.7％，两者间无显著性差异(P＞0.05)。KGF在肿瘤细胞的表达与患者的年龄、肿瘤大小、浸润程度、淋巴结转移及TNM分期均无明显相关(P＞0.05)，与分化程度密切相关，且呈显著负相关(P＜0.01)，即分化程度越低的肿瘤组织，KGF的表达率越高，分化程度越好，KGF的表达率更多还原

【Abstract】 Background: Keratinocyte growth factor (KGF) is a human mitogen that is specific for epithelial cells. It was identified in conditioned medium of a human embryonic lung fibroblast cell line by Rubin in 1989. The complementary DNA sequence of KGF demonstrates that it is a member of the fibroblast growth factor (FGF) family. It is also called FGF-7. KGF is present in stromal cells. It promotes epithelial cells mitotic division as a paracrine effector by specially binding with its high affinity receptor which is expressed on the surface of epithelial cells. The interaction of stromal and epithelial participate in the formulation and development of many tissues and organs, such as skin, gastrointestinal tract, respiratory tract and reproduction system. The interaction of stroma and tumor cells acts as an important factor during the invasion, infiltration and metastasis. In recent years we focus on the relationship between KGF and tumor.Objective: To determine the expression of keratinocyte growth factor (KGF) in human breast cancer and normal breast tissue, to investigate its correlations with clinicopathological features and some biomarkers in human breast cancer, we carried out studies to approach the clinical significance of KGF.Methods: The expression of KGF in 63 cases of breast cancer and 30 cases of normal breast tissue were detected by immunohistochemistry. We also detect the expression of estrogen receptor, progestogen receptor, CerbB-2 and Ki-67 at the same time. The correlations of the expression of KGF in tumor cells and patient’s age, tumor size, infiltration deep, lymphoid metastasis, TNM staging and some prognosis factors was analysised. The data was analysed with SPSS 12.0 for windows statistic software package.Results: Of the 63 cases of breast cancer, KGF was expressed in cytoplasm of tumor cells in 34 cases (54.0%). While it was not detected in epithelial cells in normal breast更多还原