【作者】 王振光；

【导师】 孙善珍；

【作者基本信息】 山东大学 ， 口腔基础医学， 2006， 硕士

【摘要】 第一部分 人舌鳞癌细胞系Tca-8113与正常舌黏膜成纤维细胞相互作用的实验研究 目的 初步探讨成纤维细胞与口腔癌细胞的相互作用及作用机制，为深入研究间质在口腔癌侵袭转移过程中发挥的重要作用奠定基础。 方法 采用口腔癌细胞与正常黏膜成纤维细胞直接共培养方法，模拟口腔癌的侵袭过程，在普通光学显微镜、倒置显微镜和透射电镜下观察共培养前后两种细胞的形态、超微结构变化，半定量RT-PCR法检测共培养前后与肿瘤发生发展相关的基因表达变化。 结果 两种细胞在光镜下细胞形态在共培养前后未见明显变化。在透射电镜下，共培养之后的成纤维细胞胞浆内细胞器丰富，核圆形或椭圆形一侧出现了切迹，胞膜下出现平行排列的肌丝。单独培养的Tca-8113细胞间桥粒连接常见，指状突起稀疏，细胞内可见少许溶酶体；直接共培养组的癌细胞间桥粒连接减少，细胞周缘指状突起丰富，细胞内溶酶体数量明显增多。在基因表达方面，直接共培养之后，除IGFBP1表达水平较共培养前减少(P＜0.05)外，Notch3、TFPI-2、TIMP-3、ST3、Tn-C基因表达均较共培养前增加(P＜0.05)。这些基因根据其各自的功能，分属于生长因子类／细胞因子类、信号传导／膜受体类、蛋白酶／蛋白酶抑制剂类、细胞基质及相关蛋白类，与ECM降解、血管生成、细胞生长和生存有关。更多还原

【Abstract】 Part IThe interactions between human tongue squamous cells cancer lines Tca-8113 and normal tongue mucoca fibroblastsObjective To study the interactions between human oral cancer cells and flbroblasts during oral tumor invasion and its mechanism to further explore the stroma roles in oral tumor invasion and metastasis .Methods To directly coculture human oral cancer cells with normal mucosa fibroblasts (NMFs) in vitro in order to minic oral cancer invasiveness. Two cells morphology and ultrostructures were observed under the normal microscope, inverted phase-contrast microscope and transmission electron microscope. Genes expression related to oral tumor carcinogenesis and progress was detected by semiquantitative RT-PCR before and after directly cocultured human oral cancer cells with NMFs.Results After coculture, two cells morphology were not clearly changed under the normal microscope. Fibroblasts, spindle-shaped with cytoplasmic processes, showed dentate nuclei and substantial rough endoplasmic reticulum, chondriosome and myofilament after cocultured with human oral cancer cells under更多还原