【作者】 李毅宁；

【导师】 柯晓；

【作者基本信息】 福建中医学院 ， 中西医结合临床， 2006， 硕士

【摘要】 目的：探讨慢性浅表性胃炎脾胃湿热证与热休克蛋白60（HSP60）、热休克蛋白70（HSP70）及增殖细胞核抗原（PCNA）之间的关系。 方法：慢性浅表性胃炎患者67例，其中符合中医脾胃湿热证者32例、符合脾虚证者35例，正常对照组21例，所有受试者均行胃镜检查、快速尿素酶试验及¹³C呼气试验检测Hp，胃窦部粘膜活检标本用于病理学诊断和HSP60、HSP70及PCNA的免疫组织化学染色法检测，并分析比较它们的相关性。 结果： 1．慢性浅表性胃炎脾胃湿热组Hp感染率与脾虚组相比无显著性差异（P＞0.05）；前者胃粘膜慢性炎症程度重于后者（P＜0.01）；前者胃粘膜存在活动性炎症的机率高于后者（P＜0.05）。 2．慢性浅表性胃炎脾胃湿热组和脾虚组胃粘膜HSP60的表达均强于正常对照组（P＜0.05）；脾胃湿热组HSP60的表达与脾虚组相比无显著性差异（P＞0.05）；脾胃湿热组胃粘膜HSP70的表达强于脾虚组和正常组（P＜0.05*P＜0.01）；脾虚组HSP70的表达与正常组相比无显著性差异（P＞0.05）；Hp感染与HSP60表达和HSP70表达之间呈正相关关系（P＜0.01*P＜0.05）。 3．脾胃湿热组胃粘膜PCNA的表达高于脾虚组和正常组（P＜0.05*P＜0.01）；脾虚组PCNA的表达高于正常对照组（P＜0.05）；PCNA表达与HSP70表达之间呈非常显著的正相关（P＜0.01）。 结论： 1．慢性浅表性胃炎脾胃湿热证患者胃粘膜的慢性炎症程度重于脾虚证者，处在活动性炎症阶段的机率也较大，脾虚型胃炎患者也可出现较高的Hp感染率。 2．Hp作为一种外来性的应激原其感染人胃后能刺激胃粘膜HSP60和HSP70的表更多还原

【Abstract】 Objective: To investigate the relationship between the Heat-shock Proteins 60 （HSP60）, Heat-shock Proteins 70 （HSP70）, Proliferative Cell Nuclear Antigen（PCNA） in chronic superficial gastritis patients with spleen-stomach damp-heat syndromes.Method: 67 chronic superficial gastritis patients were selected as research subjects. According to the differentiation standards of chronic superficial gastritis of TCM, 32 cases coincided to spleen-stomach damp-heat （SSDH） group, 35 cases coincided to spleen-stomach qi-deficiency （SSQD） group, and 21 healthy persons as control group. Antral mucosal biopsies were obtained during endoscopy in these patients. Rapid urease tests and ¹³C urease breath tests were used for detection of the H. pylori. The specimens of gastric antral mucosa were obtained for pathological diagnosis and immunohistochemical technique （HSP60, HSP70 and PCNA）. The correlations between every group and above index signs were analyzed.Results:1. H. pylori infection ratio was no obvious difference between the SSDH group and the SSQD group （P>0. 05） . The severity of chronic inflammation in SSDH group was higher than that in SSQD group（P<0. 01）. The ratio of active inflammation in the SSDH group were more higher than in the SSQD group （P<0.05）.2. The expression of HSP60 in chronic superficial gastritis patients with SSDH更多还原