【作者】 闫艳丽；

【导师】 熊焰；

【作者基本信息】 四川农业大学 ， 预防兽医学， 2006， 硕士

【摘要】 在不同佐剂和浓度的抗原对体液免疫、细胞免疫及仔猪生长性能影响的研究中，抗体检测结果表明，首次免疫后，免疫组抗体水平迅速升高，在第7天抗体平均OD值达到0.58，以后逐渐下降；第二次免疫后，免疫组最终稳定在0.60左右，对照组则在整个实验期间一直持续在0.46附近。这说明猪肺炎支原体MY-99株能够诱导动物机体产生良好的体液免疫应答，显示出该抗原良好的免疫原性。而不同剂量的抗原在诱导体液免疫应答上没有显示出明显的差异，抗体水平呈现相似的消长趋势；但油佐剂抗原和铝胶抗原的免疫效果间仍有微小的差异，油佐剂抗原诱导的抗体水平下降缓慢，体现出油类佐剂缓释、持久的优点。 T淋巴细胞刺激转化实验的结果显示，在免疫后的第7和14天，免疫组的T淋巴细胞转化率均在33％左右，而对照组最高为23.58％，免疫组与对照组间有明显的差异，猪肺炎支原体MY-99株具有增强动物机体细胞免疫应答的能力；不同佐剂和剂量的抗原对T淋巴细胞的刺激转化率没有差异。免疫组与对照组在生长性能上有所差异，对照组的增重速度大于免疫组(P＜0.05)，免疫组中的铝胶抗原组增重速度又大于油佐剂抗原组，但差异不显著(P＞0.05)。 对猪肺粘膜免疫系统的免疫应答的研究中，发现免疫组肺脏洗涤液中含有多量可溶性蛋白，平均为5.627mg／ml，而对照组仅为3.809mg／ml，差异极显著(P＜0.01)。免疫组和对照组肺脏均出现较为严重的肺水肿病变，对照组肺脏表面还布有出血斑点，并伴有轻度肺气肿，肺脏损伤较严重。该实验结果表明，通过肌肉注射猪肺炎支原体抗原，可以增强猪肺粘膜免疫系统的免疫应答能力，减轻肺脏损伤。 通过对仔猪体内母源抗体消长规律的研究，证实哺乳仔猪能够通过初乳从免疫母体获得高水平的被动免疫抗体，母源抗体能在仔猪体内持续30天左右，因此在仔猪断奶前的这段时期仔猪能依靠母源抗体得到被动保护。对于获得母源抗体的仔猪，在确定初次免疫日龄的实验中，我们发现在17日龄和20日龄进行初次免疫的仔猪体内，其抗体含量明显高于在11日龄和14日龄免疫的仔猪，即初次免疫的时间在仔猪出生后的2～3周进行最好，此时仔猪的免疫系统发育较完善。更多还原

【Abstract】 The study evaluated the effects of the antigen to pigs’ humoral and cellular immune responses and growth performance when using different antigen adjuvant and doses. The results of humoral immunity indicated that after the first immunifaction, antibody level of immunized pigs rose up quickly, the average OD value was 0. 58, then decreased with time;and after the second inoculation, the average OD value of immunized pigs kept more than 0.60 for a long time , while that of the control pigs’ maintained 0.46 during this period. It suggested that the antigen has good characteristic of immunogenicity. However, the effects of humoral immune responses had no difference induced by different antigen concentration, and fluctuation of the antibody levels were similar; and there was some slight difference when using different antigen adjuvant , the antibody level decreased more slowly induced by water-in-oil emulsion-adjuvant antigen than by alhydrogel-antigen antigen,which displayed slow-release and lasting characteristic of the former.An experiment about the effect of the antigen on transformation of pig peripheral blood lymphocytes indicated that the lymphocyte transformation rate of immunized pigs(35.8%) was significantly higher than control pigs(23. 58%)after immunifaction, which confirmed the antigen’s enhancement to organismal cellular immune responses;however the transformation rates have no difference when antigen adjuvant and antigen concentration were changed.Meanwhile, the control group’s growth performance was better than immunized group’ (P<0. 05) during total experiment period, and alhydrogel-antigen group better than water-in-oil-an t i gen group(P>0. 05).The second study investigated the role of immunizing intramuscularly on immune response of lung mucosal immune system. We discovered that lung washing fluid from更多还原