【作者】 龙强；

【导师】 陈伟；

【作者基本信息】 福建农林大学 ， 植物学， 2006， 硕士

【摘要】 龙眼成花逆转被认为是造成龙眼大小年结果的主要原因之一，而小年产量往往不及大年的一半，成为龙眼生产上的老大难问题。因此，进一步明确龙眼成花逆转的分子机理，阐明龙眼成花逆转过程中从基因到性状表达的规律，对于龙眼成花逆转的调控及良种培育，减少经济损失具有重要的理论和实践意义。本研究运用蛋白质组学方法和技术，通过比较龙眼成花逆转和正常发育阶段花芽的蛋白质组的变化，寻找与龙眼成花逆转紧密相关的差异蛋白，以期为今后克隆龙眼成花逆转相关基因奠定基础。 本研究以龙眼正常成花花芽蛋白质组为对照，通过双向电泳技术比较了龙眼成花逆转花芽蛋白质组的变化。比较分析2-DE图谱后发现15个差异表达的蛋白质，其中11个下调表达，4个上调表达。经LC-MS/MS串联质谱分析和数据库检索，有10个差异表达的蛋白质得到鉴定，并得到若干随机肽段的氨基酸序列信息。 与龙眼成花逆转的发生相关的10个差异蛋白中，7个下调表达，其中4个为RuBisCo大亚基，RuBisCo是植物光合作用中一个最关键的酶；1个为果糖二磷酸醛缩酶类蛋白，是植物光合作用、呼吸作用中的重要酶之一；1个为推定的花色素还原酶，与植物花色素合成和花粉育性有关；1个为NDPK Ⅰ蛋白，该酶蛋白在植物体能量代谢途径中有重要作用。3个上调表达，其中1个为推定的AP蛋白，和植物细胞衰老、凋亡相关；1个为Asb-POD酶蛋白，与植物体内自由基的清除有关；1个为无机二磷酸酶/镁离子结合/焦磷酸酶，该酶蛋白在植物体中的作用尚不清楚。更多还原

【Abstract】 Longan floral reversion is regarded as the major cause of longan poor production. As the production of "small" year is always less than half of "big" year, so longan floral reversion has become a great problem in the production of longan . Therefore, to further make sure the molecular mechanisms of longan floral reversion and the rules from genes to characters during longan floral reversion is of great theoretical and practical importance to the regulation of floral reversion, seeds breeding and reduce the economic loss.In this research, the proteome of normal flower buds was used as control. The proteomic changes of longan flower buds during floral reversion were analyzed by two-dimensional electrophoresis. 15 proteins were found to be differentially expressed, 11 of them were up-regulated and 4 were down-regulated. 10 differentially expressed proteins were identified by LOMS/MS tandem mass spectrometry and database searching. Sequences of amino acids of several random peptides were obtained.Of 10 differentially expressed proteins related to longan floral reversion, 7 were found to be down-regulated. They were 4 RuBisCo large subunit, RuBisCo is a key enzyme in plant photosynthesis; 1 Fructose-bisphosphate aldolase-like protein, this protein is an important enzyme both in photosynthesis and respiration; 1 Putative anthocyanidin reductase, it is a protein of great importance to the synthesis of anthocyanidin and the fertility of pollen. 1 NDPK I protein, it is an important protein in the energy metabolism of plants. 3 were found to be up-regulated. They are 1 Putative aspartic protease concerning to plant cell senescence and apoptosis. 1 Asb-POD protein relating to the clearance of free radicals. 1 Inorganic diphosphatase/ magnesium ion binding / pyrophosphatase, it is still a protein of unknown function in the plants.更多还原