【作者】 黄茸茸；

【导师】 李卫平； 明亮；

【作者基本信息】 安徽医科大学 ， 药理学， 2006， 硕士

【摘要】 缺血性脑血管疾病是临床上常见病，研究缺血性脑血管疾病的发病机理和防治缺血性脑血管疾病损伤的方法已成为神经科学领域研究的热点和难点。黄芪有补中益气、扶正固本等功效，单味黄芪及复方是治疗肝、肾疾病和恶性肿瘤以及抗衰老和促智方面的传统中药，也是中医药防治缺血性脑卒中疾病最常用药物之一。本课题组多年研究黄芪有效成分的药理作用，发现黄芪总苷(Astragalosides，AST)和黄芪多糖(Astragalus Polysaccharides，APS)具有调节机体免疫功能、提高机体抗氧化能力、抗肿瘤以及抗胶原增殖等作用。黄芪提取物(Extract of Astragalus，EA)为黄芪的有效部位由黄芪总苷和黄芪多糖组成。本文在我们前期研究工作的基础上，和用线栓法制备的大鼠局灶性脑缺血(大脑中动脉栓塞，MCAO)再灌注模型，观察了EA防治缺血性脑血管疾病的作用，并探讨了EA对局脑缺血再灌注损伤的保护作用是否与其调节机体炎症免疫反应功能有关。 1 EA对大鼠局脑缺血再灌注损伤的保护作用 在用Bederson法对局脑缺血再灌注大鼠的神经功能障碍评分实验中，EA80mg／kg组可改善2h的神经功能障碍，EA(40、80mg／kg)组可降低8h的神经功能评分，三个剂量组均可明显改善24h的神经功能障碍。在对局脑缺血再灌注大鼠的脑含水量和脑梗死体积的测定中，EA(20，40，80 mg／kg)组均能显著减轻脑缺血再灌注后的脑组织含水量增加，同时能明显减小脑缺血再灌注后大鼠的脑梗死体积。表明EA对大鼠局脑缺血再灌注损伤具有较好的保护作用。 2 EA对大鼠局脑缺血再灌注血清和脑组织中TNF-a、IL-1β及IL-6等的影响 在大鼠局脑缺血(MCAO)2h再灌注24h实验中，EA(40、80mg／kg)能降低血浆循环内皮细咆(CEC)数量；EA(40、80mg／kg)能降低外周血WBC总数，EA(20、40、80mg／kg)还能降低嗜中性粒细胞百分率，升高淋巴细胞百分更多还原

【Abstract】 Astragalus is one of traditional Chinese medicine against cerebral ischemia and the extract of astragalus (EA) is effective components of astragalus which is mainly composed of astragalosides and astragalus polysaccharides.Our earlier research indicated that EA had better protective effects on cerebral ischemia and also had better antioxidation , anti-inflammation and immunoregulation and others pharmacology functions . Middle cerebral artery occlusion(MCAO) was used to make local cerebral ischemia-reperfusion model in rats with intravascular nylon filament occlusion in this paper. Besides we also futher investigated the correlation between the protective effects and its mechanisms of the extract of astragalus (EA) against local cerebral ischemia-reperfusion injury with the anti-inflammation and lmmunoregulation.The main results are showed as follows:1 Protective effects of EA on local cerebral ischemia and reperfusion injury in ratsThis experiment was performed to evaluate the neurological function disorder by Bederson .EA 80mg/kgs EA (40 、 80mg/kg) and EA (20 、 40、 80mg/kg) could respectively decrease the neurological function score for 2s 8 and 24h.EA (20、 40、 80mg/kg ) could reduce the brain edema obviously and lessen histopathological alterations of cortex in brain sections.2 Effects of EA on the inflammatory response and immunoregulation during local cerebral ischemia and reperfusion injury in ratsThis experiment was performed to evaluate effects of EA on local cerebral ischemia injury induced by middle cerebral artery occlusion (MCAO) for 2h and reperfusion for 24h in rats. EA (40、 80mg/kg) could inhibit the increase of circulating endothelial cells number in plasma and also could decrease the counts of WBC in blood; EA (20、 40、 80mg/kg) increased the neutrophil percentage and decreased the T更多还原