【作者】 滕丽萍；

【导师】 德伟；

【作者基本信息】 南京医科大学 ， 生物化学与分子生物学， 2006， 硕士

【摘要】 运用高密度寡和苷酸芯片建立大鼠胰腺发育不同阶段(胚胎(E)12.5，E15.5，E18.5，新生和成年)的基因表达谱，经过生物信息学数据分析发现许多与胰腺功能相关的基因在E18.5相对高表达，提示胚胎发育后期胰腺功能正趋于完善和成熟，这个时期正是胰岛细胞迁移聚集形成典型胰岛结构的关键阶段，而典型的胰岛结构是胰腺发挥正常生物学功能的基础。本研究从基因表达谱中筛选出一条在E18.5特异性高表达的基因Mesothelin，旨在初步探讨该基因在胰岛细胞迁移、黏附及胰岛结构维持中的作用。 运用实时荧光定量PCR技术进一步验证Mesothelin基因在大鼠胰腺胚胎发育不同阶段的mRNA表达水平，同时检测与之相关的上游调控因子Wnt-1和Wnt-5a的表达情况。结果显示：实时荧光定量PCR与基因芯片中检测到的Mesothelin基因的表达趋势一致；Wnt-5a呈现出两种截然相反的表达趋势，其中一种趋势与基因芯片中的表达相吻合，且与Mesothelin的表达趋势相反；Wnt-1表达量极低，几乎未检测到。 基于以上实验结果，我们推测细胞黏附分子Mesothelin可能调控胰腺胚胎发育后期胰岛细胞的迁移、聚集、黏附及胰岛结构的形成和更多还原

【Abstract】 We expect to use high-density microarray (Affymetrix) to analyze the expression profiles of pancreas development. We generated transcriptional profiles of pancreatic tissue isolated from five biologically significant stages of development: embryonic day (E) 12.5, E15.5, E18.5, newborn and adult pancreas. These analyses implicated that many genes such as Mesothelin were enriched in the later gestation when islet architecture and function are gradually forming. The differential expression of Mesothelin and its upstream regulator-Wnts were verified by real-time PCR. Moreover, the result of real-time PCR indicated that perhaps Wnt-5a has transcript variants. Our present preliminary evidence suggested that Mesothelin might be a novel regulator of islet formation and maturation. In addition, transcript variants of Wnt-5a probably play a role in pancreas development.更多还原