【作者】 马震宇；

【导师】 邢春根；

【作者基本信息】 苏州大学 ， 普外科， 2006， 硕士

【摘要】 第一部分 大肠癌肝转移模型建立的方法学改进 目的：建立模拟临床大肠癌根治术后肝转移动物模型，并探讨模型建立方法的改进。方法：在裸鼠脾脏内注入人结肠癌细胞5min后切除脾脏。术后分别于20、35、60天对裸鼠行病理检查。结果：发现肝转移率为90％，肝外其它脏器未见转移结节，组织学上证实肝转移结节为低分化腺癌。结论：本实验方法建立的模型能很好地模拟临床大肠癌根治术后肝转移征象，同时在麻醉药物及浓度选择、裸鼠的固定、手术切口部位及长度、细胞悬液注射的部位及速度、脾脏切除的注意事项和术后的观察等方面做了改进。方法的改进提高了模型荷瘤的成功率和器官靶向性。 第二部分 甲氧雌二醇对大肠癌肝转移的抑瘤作用 目的：实验研究血管生成抑制剂甲氧雌二醇(2-methoxyestradiol 2-ME)对大肠癌肝转移的抑制作用。方法：采用人大肠低分化腺癌细胞株LS174T注射入裸鼠脾脏，后将脾脏切除建立类似于临床的大肠癌肝转移裸鼠模型。建模后第3周开始分组治疗，分别腹腔注射PBS100mg／kg(对照组)、2-ME 50mg／kg、2-ME 100mg／kg、2-ME 150mg／kg、5-FU50mg／kg(治疗组)。第7周处死动物，测量肝脏转移肿瘤瘤重、肿瘤微血管密度(microvessel density，MVD)、抑瘤率、肿瘤细胞的凋亡指数(apoptotic index，AI)。结果：肝脏转移肿瘤瘤重依次为：(1.41±0.39)g、(1.03±0.27)g、(0.79±0.12)g、(0.54±0.21)g、(0.67±0.14)g；MVD依次为：(13.8±5.2)、(9.1±2.7)、(3.3±1.3)、(0.85±0.6)、(8.4±3.4)；抑瘤率依次为：0％、26.95％、43.97％、61.70％、52.48％；AI依次为：(3.6±2.4)％、(5.4±1.7)％、(9.1±3.1)％、(12.8±3.9)％、更多还原

【Abstract】 Part I: The improvement in establishment of a model of human colon cancer liver metastases in nude miceObjective : To establish an animal model imitating clinic liver metastases after curative resection of colorectal cancer and discuss the improvement in establishment of the model . Methods: The human colon cancer cells(HCCC)were injected into the spleen of BALB/c nude mice , then the spleen were resected 5 minutes later. On the20th、 35th、 60th postoperative day, The nude mice were examined pathologically. Results: The incidence of liver metastases was found 90%.No metastases noudle was observed in extrahepatic organs. Histological examination showed that the metastases were poorly differented colon adenocancer. Conclusion: The experimental model is very good for imitating clinic sign of liver metastases after curative resection of colorectal cancer. At the same time, several improvement were made in the establishment of the model.PartII: Experimental study of the inhibit effects of 2-methoxyestradiol(2-ME) on the liver metastases of colon cancerObjective: In this study,we aimed to investigate the effects of angiogenesis inhibitor 2-methoxyestradiol(2-ME)on the liver metastases of更多还原