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用DNA序列探讨壁虎科蜥蜴间的关系和云南半叶趾虎的种群结构
Interrelationships among Gekkonidae Lizards and Population Structure of Hemiphyllodactylus Yunnanensis Inferred from DNA Sequences
【作者】 冯金叶;
【导师】 周开亚;
【作者基本信息】 南京师范大学 , 动物学, 2006, 硕士
【摘要】 基于12S rRNA基因全序列及C-mos基因序列片段探讨了壁虎科(Gekkonidae)蜥蜴的属间关系;用Cyt b基因全序列和ND2-tRNATyr基因片段初步研究了云南半叶趾虎(Hemiphyllodactylus yunnanensis)的种群结构;用12S rRNA基因全序列探讨了中国壁虎属(Gekko)七物种间的系统关系。 1 壁虎科蜥蜴的属间关系 壁虎科包括了壁虎类蜥蜴85%的属,广泛分布于所有大陆及几乎全部岛屿。到目前为止,壁虎科是壁虎类蜥蜴中属间关系最不清楚的一个分支。本研究获得了壁虎科34个属和鳞脚虎科2个属的C-mos基因部分序列和线粒体12S rRNA基因全序列。最大简约法(MP)和最大似然法(ML)用软件PAUP 4.0b10进行分析。贝叶斯法分析应用MrBayes 3.0B4软件。按照多数一致原则(>50%)构建一致树。所有的系统发生树都支持壁虎科是单系的。在一致树中,壁虎科共分为A-F六个支,这六个分支与传统的形态学分组之间有着较大的差异。从构建的系统发生树中可以发现Sphaerodactylus位于壁虎亚科的内部。结果同时也显示除Ailuronyx之外,Teratoscincus+Pristurus是壁虎科其他壁虎的姐妹群,这在一定程度上证明了沙虎亚科的有效性。因此将球趾虎族提升为球趾虎亚科,还是维持Kluge(1987)的球趾虎族,以及沙虎亚科的有效性问题还值得进一步研究。 2 云南半叶趾虎的种群结构
【Abstract】 In the present study C-mos nuclear and 12S rRNA mitochondrial DNA were employed as genetic markers to deduce the interrelationships among Gekkonidae lizards; Cyt b and ND2- tRNATyr gene sequences were used to discuss the population structure of Hemiphyllodactylus yunnanemis; 12S rRNA gene sequences were used to reveal the phylogenetic relationships among seven species of the genus Gekko.1 Interrelationships among genera of GekkonidaeGekkonidae lizards, representing more than 85 percent of the Gekkotan genera,are found on all major land masses and almost all oceanic islands. Intergenericrelationships of the Gekkonidae have been far more difficult to resolve than thoseamong other gekkotan families. Our data set consisted of a large number of completemitochondrial 12S rRNA gene sequences and partial nuclear C-mos gene sequencesfor 34 genera of geckos and 2 genera of pygopods. Maximum parsimony (MP) andmaximum likelihood (ML) trees were generated based on unweighted analysis usingPAUP 4.0b10. Bayesian inference (BI) analyses trees were generated by MrBayes3.0B4. A majority-rule (>50%) consensus tree was constructed. All phylogenetic treessupported the monophyly of Gekkonidae with great confidence. There were six
【Key words】 Gekkonidae lizard; molecular phylogeny; Hemiphyllodactylus yunnanensis; population structure; Gekko; C-mos; 12S rRNA; Cyt b; ND2-tRNATyr;
- 【网络出版投稿人】 南京师范大学 【网络出版年期】2006年 12期
- 【分类号】Q951;Q953
- 【被引频次】2
- 【下载频次】198