【作者】 董大鹏；

【导师】 张学俊；

【作者基本信息】 贵州大学 ， 发酵工程， 2006， 硕士

【摘要】 近20年来，活性氧和自由基研究成为现代生命科学的前沿和热点，评价和筛选具有强抗氧化活性的天然资源己成为生物学、医学和食品科学研究的新趋势。杜仲具有优良的抗自由基、抗氧化、抗衰老、及降血压等方面的生物学功效，近年来研究表明，杜仲叶与皮的有效成分基本相同。因此从丰富的杜仲叶资源中提取分离抗氧化活性成分具有重要意义。 本文在详细总结了目前杜仲叶活性成分提取与分离的基础上，对用乙醇溶液提取和大孔树脂分离法进行了深入研究。通过单因素试验对提取溶剂、提取时间、料液比和提取次数进行考察，确定了最佳提取工艺。在抗氧化活性成分分离富集时，采用了对环境和人体无毒害作用的树脂吸附工艺，通过对三种不同树脂对抗氧化活性成分的研究，筛选出X-5型树脂用于快速分离技术，得到很理想杜仲叶提取液中抗氧化活性样品，主要结果如下： 在超声波助溶，提高胞内物质传递的条件下，当料液比为1：18，溶剂为含20％乙醇的水溶液时，能够最大效率地加速杜仲叶植物纤维的润涨作用，提高抗氧化物质的溶出率，研究还表明，每次经过40min的超声处理，提取2次，即可达到93％以上的提取效率。通过静态吸附和动态吸附性能比较，以及静态吸附动力学曲线筛选出对杜仲叶抗氧化活性物质吸附性能最好的大孔吸附树脂为X-5，最佳解吸剂为70％乙醇溶液。以TAC为活性跟踪指标，经X-5大孔树脂吸附快速分离纯化后筛选出抗氧化能力较强的一组，其TAC可提高4.83倍，表明用大孔吸附树脂对杜仲抗氧化提取物进行纯化的效果是较理想的。 本研究为充分利用杜仲叶资源开发提供了新方法和新思路。更多还原

【Abstract】 In the last 20 years, active oxygen and free radical have become the hot topics in modern life science, and appraisal and selection of natural resources with strong antioxidative activities have become the new trends of biology, medical science and food science and technology.Eucommia Ulmoides Oliv has many wonderful functions, such as anti-free radical, oxidation resistance and decrease blood pressure etc. It has been found that the leaves contain almost the same effective components as the bark. So it was economic significations that antioxidative are extracted from a great deal of leaves of Eucommia Ulmoides Oliv and purified activesIn this paper, extraction and separation of antioxidant actives from the leaves of Eucommia Ulmoides Oliv were reviewed at first. Alcohol-extraction and then resin adsorption method were further studied. Through investigating the effects of extractive solution, extractive time, ratio of solvent to material and extraction times on TAC, the optimum condition are concluded. At the same time, Resin adsorption method was used to isolate and enrich antioxidant actives from the extraction solution. By comparing four different macro-porous resins in separating capacity, X-5 was the best one in separating antioxidant activities.The results suggested that in the condition of the ratio 1:18, a mixed solution containing 20 % (v/v) of ethanol speeded up the solvent-swell of leaves and promoted the natural compounds transferring and increased the TAC (Total Antioxidative Capacity). Experiments showed the extraction rate under ultrasonic wave for twice, 40 min was over 93%. By comparing static and dynamic adsorption performance, macropore adsorptive resin X-5 and optimum eluant 70% ethanol solution, were selected, After adsorption and purification on column, TAC of antioxidative extract of Eucommia Ulmoides Oliv was increased by 4.83 times. Resin X-5 can be used to purify antioxidative actives from abundant更多还原