共代谢对难降解有机物生物降解性能的影响

【作者】 姚珺；

【导师】 何占航；

【作者基本信息】 郑州大学 ， 化学， 2006， 硕士

【摘要】 难降解有机物容易在环境中积累，其中大多数种类对人体和其他生物有害。难降解有机物中多环芳烃和杂环化合物大多对生物具有致癌、致畸变作用。本文以多环芳烃化合物（萘、蒽、联苯、三联苯）和杂环化合物（吡咯、呋喃、咪唑、吡啶、喹啉、吲哚、咔唑、吩噻嗪）为对象，研究了微生物共代谢作用对其生物降解性能的影响。试验结果表明：（1）在受试物单一基质条件下，呋喃和萘可生物降解，吡咯、吡啶、咪唑、喹啉、吲哚、蒽难于生物降解。联苯、三联苯、咔唑、吩噻嗪对微生物细胞有抑制作用。当用苯酚作为一级基质，受试物作为二级基质，在共代谢作用下，吡咯、咪唑、喹啉、吲哚、蒽生物降解性能均有所改善，但是吡啶、咔唑、吩噻嗪、联苯、三联苯仍为难降解有机物。（2）微生物共代谢同样存在于厌氧微生物对有机物的降解中。受试物经过厌氧酸化后，其好氧生物降解性能均有不同程度的改善。（3）微生物共代谢中，采用不同种类的一级基质，对难降解有机物生物降解性能的影响是不同的。（4）共代谢中一级基质葡萄糖的浓度可以影响到受试有机物的生物降解性能，影响效果遵循公式：E-E₀=ΔE_maxBOD₅/（BOD₅+K_b）更多还原

【Abstract】 Refractory organic can be accumulated in the environment easily , most of them is harmful to the human being and other beings. Among the total ,most of the polycyclic aromatic hydrocarbon and heterocyclic compound have the carcinogenic action and teratogenesis to the organism.This paper studied the effect of co-metabolism on the biodegradability of the PAHs and heterocyclic compounds. The compounds including naphthalene, anthracene,diphenyl,terphenyl,pyrro,furan,imidazole,pyridine,quinoline,indole,carbaz ole,phenothiazine.The experimental results are as follow:(1) Under the sole-substrate condition ,furan and naphthalene can be degraded ,pyrro,imidazole,pyridine, anthracene, quinoline,indole can be degraded difficulty, diphenyl, terphenyl, carbazole,phenothiazine inhibit microorganism. The aerobic biodegradability of the compounds such as pyrro, imidazole, quinoline,indole, anthracene are improved when phenol as the primary substrate and the tested compounds as the secondary substrate. In these condition , diphenyl, terphenyl,, pyridine, carbazole, phenothiazine cann’t be bio-degraded.(2) Cometabolism is existed under anaerobic condition , the aerobic degradation of the tested pollutants are improved to varying degrees after anaerobic acidification.(3) In the co-metabolism ,the effect of varying primary substrate on the bio-degradability of the refractory organic is different.(4) In the co-metabolism, the bio-oxidation ratio of tested organics can be affected by concentration of the primary substrates. The effect of influence followthe formula:更多还原