【作者】 祁颖；

【导师】 张凤妍；

【作者基本信息】 郑州大学 ， 眼科学， 2006， 硕士

【摘要】 目的 研究榄香稀(Elemene，Ele)对体外培养的兔晶状体上皮细胞(rabbit lens epithelial cells，RLECs)形态、增生的影响，为筛选治疗后发性白内障的药物提供依据。 方法 首先进行兔晶状体上皮细胞体外培养，观察兔晶状体上皮细胞的形态学变化和增生规律。在第2、3、4代RLECs中加入不同剂量的榄香稀，作用不同时间，观察榄香稀对兔晶状体上皮细胞增生的影响。 结果 1．细胞计数法显示细胞在2—7天处于生长对数期，7天达到最高峰。 2．榄香稀能够抑制兔晶状体上皮细胞的增生，其抑制作用随着药物浓度的增加、作用时间的延长而增强。 结论 1．榄香稀能够抑制兔晶状体上皮细胞的增生，其抑制作用随着药物浓度的增加、作用时间的延长而增强。 2．榄香稀可能成为防治后发性白内障的药物。更多还原

【Abstract】 Objective: To study the effect of Elemene on the proliferation of the rabbit lens epithelial cells (RLECs) in vitro and to evaluate the drugs used for posterior capsular opacification (PCO) after cataract extration.Method: (1) RLECs were cultured in vitro and we observed cells morphological changes with light microscope to calculate the cells growth regulation. (2) Elemene of different concentrations was added to RLECs of the second, third, fourth generation. The effect of elemene on rabbbit lens epithelial cells in vitro was observed.Results: (1) With the cells counting method, the cells growth was in logarithm growth stage on the second to seventh day. The peak of growth was on the seventh day. (2) Elemene could significantly inhibit the proliferation of RLECs. With the increasement of dose and time, the inhibitory rate increased.Conclusion: (1) Elemene could significantly inhibit the proliferation of RLECs. With the increasement of dose and time, the inhibitory rate increased. (2) Elemene might be a candidate to prevent the posterior capsular opacification .更多还原