【作者】 刘丽霞；

【导师】 孔繁斗；

【作者基本信息】 大连医科大学 ， 妇产科学， 2006， 硕士

【摘要】 目的:卵巢癌患者病死率居妇科肿瘤之首,且发病率近年来有上升趋势,其总体5年生存率为30%左右。不同期别患者预后差异很大。导致卵巢癌患者死亡的原因并不限于原发灶本身,而常在于其产生的远处转移和复发。约3/4的患者就诊时已为III-IV期,主要由于缺乏有效的检测肿瘤转移的方法。因此,通过有效的诊断指标来检测那些有远处转移和复发的高危患者,对他们进行有效的综合治疗,能明显提高其生存率和生存期,改善卵巢癌患者的生存质量,降低死亡率。近年来,多项研究显示,骨桥蛋白与其配体在多种肿瘤上表达,骨桥蛋白(osteopontin,OPN)通过激活CD44v6介导的信号传导通路,促进细胞存活、趋化、归巢、黏附,提高肿瘤的侵袭能力。目前,关于OPN与CD44v6分子在卵巢癌中共同表达与卵巢癌关系的报道甚少,尚处于探索之中,本实验采用RT-PCR方法,探讨OPN与CD44v6分子mRNA在卵巢癌中的表达情况及其与卵巢肿瘤临床病理特征之间的关系,并分析OPN与CD44v6之间的相关性,进一步阐明OPN和CD44v6分子在卵巢癌侵袭和转移过程中的作用。方法:应用逆转录聚合酶链反应(RT-PCR)方法,检测OPN和CD44v6 mRNA在卵巢癌组织中的表达和相对含量,分析其与卵巢癌临床病理特征的关系。结果: OPN和CD44v6 mRNA的表达与肿瘤临床分期、组织学分级、淋巴结转移与否具有显著性差异(P<0.05 or P<0.01),与患者的年龄、肿瘤的病理类型无显著性差异(P>0.05); OPN和CD44v6间相比较,有显著相关性(r=0.35 , P<0.01)。结论:1、OPN与CD44v6 mRNA的表达增高,在上皮性卵巢癌更多还原

【Abstract】 Objective: Ovarian cancer is the first case fatality ratio in gynecological malignancies, and its incidence shows a upgrade tendency. The total 5 years survival rate is about 45% and there is a significant prognosis between difference grades. The main reasons leading to death are the metastasis and recurrence of tumors. Three quarters of patients present with International Federation of Gynecology and Obstetrics stage III-IV disease, chiefly because there is no reliable screening technique for invasion and metastasis. So markers with a well-defined and validated role in monitoring of cancer metastasis are important. By this way, we can reduce the death rate and improve the living quality. Recently, many studies have shown that, osteopontin(OPN) can interact with CD44v6,and expressed in numerous human cancers. OPN activates CD44v6 mediated pathways to promote cell survival, chemotaxis, homing and adhesion, each potentially enhancing metastatic behavior in tumors. To investigate the correlation between osteopontin and CD44v6 mRNA with clinicopathologic features of ovarian tumor ,we used semi-quantitive RT-PCR to detect the mRNA expression of OPN and CD44v6 and elucidate their role in tumor invasion and distant metastasis.Methods: The expression of OPN and CD44v6 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). The relationship between the relative content of OPN and CD44v6 mRNA and clinicopathologic features of ovarian cancer was analyzed.Results: The was statistically correlated with histology classification of OC(P<0.05).The OPN and CD44v6 expression in advanced(stage III-IV) OC were higher than that of initial stage(I-II) (P<0.05),as well as the更多还原