【作者】 刘芳；

【导师】 马国武；

【作者基本信息】 大连医科大学 ， 口腔基础医学， 2006， 硕士

【摘要】 背景:口腔鳞状细胞癌(oral squamous cell carcinoma OSCC)是口腔中最为常见的恶性肿瘤,占口腔颌面部恶性肿瘤的80%以上,研究OSCC的发生发展,对OSCC做出早期的诊断对临床工作有重要的指导意义。生存素Survivin是新近发现的一种凋亡抑制基因,其表达的蛋白是凋亡抑制蛋白家族的新成员,具有抑制细胞凋亡的作用,并在细胞的有丝分裂及胞质分裂过程中起着重要的作用。研究表明survivin是一个肿瘤特异性的凋亡抑制基因,在胚胎发育过程中和人的肿瘤组织中表达,而在成熟的终末分化组织中表达降低或缺失。增殖细胞核抗原(PCNA)是DNA聚合酶δ的辅助因子,参与DNA的合成,在细胞周期调控中起重要作用。PCNA的周期性变化与细胞增殖过程有关,在调节细胞增殖和恶性疾病的发生发展中起重要作用。目的:探讨survivin在口腔鳞状细胞癌发生发展中的作用及其与细胞增殖的关系。通过对survivin在口腔鳞状细胞癌中表达情况及其与细胞增殖活性关系的研究,分析survivin参与癌变发生的分子机制,为临床提供有价值的早期诊断和预后指标,为有效治疗口腔鳞状细胞癌提供新的靶基因。材料和方法:1.选取以4NQO诱发的小鼠舌癌动物模型标本111例,所有标本均经病理诊断证实,其中对照组10例,不典型增生80例(其中轻度5例,中度30例,重度45例),口腔鳞状细胞癌21例(其中原位癌13例,早期浸润癌8例)全部组织经10%中性福尔马林固定,石蜡包埋。2.应用免疫组织化学S-P法检测Survivin和PCNA的表达情况。3.统计分析结果。更多还原

【Abstract】 BackgroundSquamous cell carcinoma of oral (OSCC) is the most common malignant tumour in the oral cavity, accounting for more than 80% of the oral malignant tumour.However,study the process of OSCC,making early correct diagnoses on OSCC have important directive signifcance to clinical work.Survivin is a new antiapoptosis gene, which is discovered recently, and survivin protein is a new member of inhibition of apoptosis protein(IAP) family. It can inhibit cell apoptosis, and play a significant role in cell mitosis and cytoplastic cleavage. Studys suggest that survivin is a tumor specific apoptosis gene, it is expressed during the fetal development and in common human tumors, but cannot be found in normal adult tissues. Proliferating cell nuclear antigen(PCNA) is a assistant factor of DNA polymerase 8, participating in DNA synthesize, and playing a great role in regulation of cell cycle. The cyclic change of PCNA is related to cell proliferating process, it’s a milestone during the period of cell proliferation and malignant disease.ObjectiveThe aim is to investigate the function of survivin in oral squamous cell carcinoma of oral (OSCC) and evolution, and its relation to cell proliferation. Through the study of survivin expression in OSCC and its relation to cell proliferating activity, we analyzed the molecular mechanism of survivin during oncogenesis. It may be a valuable target for early diagnosis and therapy of OSCC,and can provide a new target gene for effectual remedy to OSCC.更多还原